The impact of gestational diabetes on changes in the concentration of selected hormones regulating food intake in umbilical cord blood, and the development of obesity in children in later life
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Department of Mother and Child Health, University of Medical Sciences, Poznań, Poland
Department of Medicine, President Stanisław Wojciechowski State University of Applied Sciences, Kalisz, Poland
Department of Animal Physiology, Biochemistry and Biostructure, University of Life Sciences, Poznań Poland
Students’ Scientific Society, President Stanisław Wojciechowski State University of Applied Sciences, Kalisz, Poland
Collegium Medicum, Institute of Health Sciences, University of Zielona Góra, Poland
Department of Anaesthesiology and Intensive Care, Hospital of the Ministry of the Internal Affairs and Administration, Poznań, Poland
Corresponding author
Paweł A Kołodziejski   

Department of Animal Physiology, Biochemistry and Biostructure, University of Life Sciences, Poznań, Poland
J Pre Clin Clin Res. 2023;17(3):149–156
Introduction and objective:
Gestational diabetes mellitus (GDM) is a metabolic disease affecting an increasing number of women. The disease not only affects the health of mothers, but may also contribute to metabolic problems in children, both at the stage of foetal life and in the future, including increasing the risk of obesity or type 2 diabetes. Therefore, it is important to conduct research to get closer to fully defining the problem and developing therapies or methods of preventing GDM

Material and methods:
The study used umbilical cord blood from 540 women (260- healthy and 280 – GDM) and commercially available ELISA and RIA tests to investigate the interaction between leptin, ghrelin (active and total) and insulin in cord blood, and its correlation with anthropometric parameters of newborns as well as pregnancy week on the day of delivery, weight, BMI and growth of mothers.

It was found that in healthy mothers there were statistically significant correlations between maternal pre-pregnancy weight in relation to leptin (positive) and glucose (negative), a positive correlation between active ghrelin concentration and gestational week at delivery, and a positive level of leptin to the chest circumference of newborns, and a negative of glucose levels to the chest circumference of newborns at birth in the healthy group. In the GDM group, a positive correlation was found of total ghrelin to the abdominal circumference of the newborns at birth, and a positive of leptin concentration and thigh circumference of the newborns

It was concluded that altered hormonal profiles during the prenatal period may have long-term consequences for the health of children. The study also indicated the need for further research in this area to better understand the causes and consequences of GDM r.

Wojciechowska M, Chęcińska-Maciejewska Z, Pruszyńska-Oszmałek E, Ciborek A, Gibas-Dorna M, Rękas-Dudziak A, Krauss H, Kołodziejski PA. The impact of gestational diabetes on changes in the concentration of selected hormones regulating food intake in umbilical cord blood, and the development of obesity in children in later life. J Pre-Clin Clin Res. 2023; 17(3): 149–156. doi: 10.26444/jpccr/172938
Buchanan TA, Xiang AH, Page KA. Gestational diabetes mellitus: Risks and management during and after pregnancy. Nat Rev Endocrinol. 2012;8(11):639–649.
Xiang AH, Wang X, Martinez MP, Getahun D, Page KA, Buchanan TA, et al. Maternal gestational diabetes mellitus, type 1 diabetes, and type 2 diabetes during pregnancy and risk of ADHD in offspring. Diabetes Care. 2018;41(12):2502–2508.
Yavuzkir S, Ugur K, Deniz R, Ustebay DU, Mirzaoglu M, Yardim M, et al. Maternal and umbilical cord blood subfatin and spexin levels in patients with gestational diabetes mellitus. Peptides (NY). 2020;126: 170277.
Yuan J, Zhang D, Wang Y, Zhu Z, Lin Q, Li M, et al. Angiopoietin- Like 8 in Gestational Diabetes Mellitus: Reduced Levels in Third Trimester Maternal Serum and Placenta, Increased Levels in Cord Blood Serum. Int J Endocrinol. 2022;26;2022:1113811. https://doi. org/10.1155/2022/1113811.
Lende M, Rijhsinghani A. Gestational diabetes: Overview with emphasis on medical management. Vol. 17, International Journal of Environmental Research and Public Health. 2020;21;17(24):9573. https://
Perboni S, Inui A. Appetite and gastrointestinal motility: Role of ghrelin-family peptides. Clinical Nutrition. 2010;29(2):227–34. https://
Izquierdo AG, Crujeiras AB, Casanueva FF, Carreira MC. Leptin, obesity, and leptin resistance: where are we 25 years later? Nutrients. 2019;11(11):1–11.
Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman J. Positional cloning of the mouse obese gene and its human homologue. Nature. 1994;372:425–432.
Isse N, Ogawa Y, Tamura N, Masuzaki H, Mori K, Okazaki T, et al. Structural Organization and Chromosomal Assignment of the Human obese Gene. J Biol Chem. 1995;270(46):27728–27733. S0888754385710166.
Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature. 1999;402(6762):656–60.
Daniels TE, Mathis KJ, Gobin AP, Lewis-de los Angeles WW, Smith EM, Chanthrakumar P, et al. Associations of early life stress with leptin and ghrelin in healthy young adults. Psychoneuroendocrinology. 2023;149: 106007.
Morante JJH, Soler ID, Muñoz JSG, Sánchez HP, Ortega MDCB, Martínez CM, et al. Moderate weight loss modifies leptin and ghrelin synthesis rhythms but not the subjective sensations of appetite in obesity patients. Nutrients. 2020;12(4):916.
Harreiter J, Simmons D, Desoye G, Corcoy R, Adelantado JM, Devlieger R, et al. IADPSG and WHO 2013 gestational diabetes mellitus criteria identify obese women with marked insulin resistance in early pregnancy. Vol. 39, Diabetes Care. 2016;39(7):e90–2. 0200.
Cypryk K. Cukrzyca ciążowa — rozpoznawanie i leczenie. Ginekologia i Perinatologia Praktyczna. 2016;1(2):41–44.
Wender-Ożegowska E, Bomba-Opoń D, Brązert J, Celewicz Z, Czajkowski K, Gutaj P, et al. Standardy Polskiego Towarzystwa. Ginekologów i Położników postępowania u kobiet z cukrzycą. Ginekologia i Perinatologia Praktyczna. 2017;2(5):215–229.
Mitanchez D, Yzydorczyk C, Siddeek B, Boubred F, Benahmed M, Simeoni U. The offspring of the diabetic mother – Short- and long-term implications. Best Pract Res Clin Obstet Gynaecol. 2015;29(2): 256–69.
Pedersen J. Weight and length at birth of infants of diabetic mothers. Acta Endocrinol (Copenh). 1954;16(4): 553–554. acta.0.0160330.
Freinkel N. Banting Lecture 1980: of Pregnancy and Progeny. Diabetes. 1980;29(12):1023–35. /diab.29.12.1023.
Johns EC, Denison FC, Norman JE, Reynolds RM. Gestational Diabetes Mellitus: Mechanisms, Treatment, and Complications. Vol. 29, Trends in Endocrinology and Metabolism. 2018;29(11):743–754. https://doi. org/10.1016/j.tem.2018.09.004.
Wójcik M, Chmielewska-Kassassir M, Grzywnowicz K, Woźniak L, Cypryk K. The relationship between adipose tissue-derived hormones and gestational diabetes mellitus (GDM). Vol. 65, Endokrynol Pol.2014;65(2):134–42. /EP.2014.0019.
Liu X, Zheng T, Xu YJ, Yang MN, Wang WJ, Huang R, et al. Sex Dimorphic Associations of Gestational Diabetes Mellitus With Cord Plasma Fatty Acid Binding Protein 4 and Estradiol. Front Endocrinol (Lausanne). 2021;12:740902.
Pettitt DJ, Baird HR, Aleck KA, Bennett PH, Knowler WC. Excessive Obesity in Offspring of Pima Indian Women with Diabetes during Pregnancy. N Engl J Med.1983;308(5):242–5. nejm198302033080502.
Bashir IA, Mariod AA, Banu R, Elyas TB. Significance of health related predictors of diabetes in Pima Indians women. Curr Res Nutr Food Sci. 2019;7(2):350–359.
Silverman BL, Rizzo T, Green OC, Cho NH, Winter RJ, Ogata ES, et al. Long-term prospective evaluation of offspring of diabetic mothers. Diabetes. 1991;40(2):121–5.
Gillman MW, Rifas-Shiman S, Berkey CS, Field AE, Colditz GA. Maternal gestational diabetes, birth weight, and adolescent obesity. Pediatrics. 2003;111(3):221–226.
Dugas C, Perron J, Kearney M, Mercier R, Tchernof A, Marc I, et al. Postnatal Prevention of Childhood Obesity in Offspring Prenatally Exposed to Gestational Diabetes mellitus: Where Are We Now? Vol 10, Obesity Facts. 2017;10(4):396–406.
Zhao P, Liu E, Qiao Y, Katzmarzyk PT, Chaput JP, Fogelholm M, et al. Maternal gestational diabetes and childhood obesity at age 9–11: results of a multinational study. Diabetologia. 2016;59(11):2339–2348. https://
Mendoza-Herrera K, Florio AA, Moore M, Marrero A, Tamez M, Bhupathiraju SN, et al. The Leptin System and Diet: A Mini Review of the Current Evidence. Vol. 12, Front. Endocrinol. 2021;24(12):749050.
Obradovic M, Sudar-Milovanovic E, Soskic S, Essack M, Arya S, Stewart AJ, et al. Leptin and Obesity: Role and Clinical Implication. Vol. 12, Front. Endocrinol. 2021;18(12):585887. fendo.2021.585887.
Chou SH, Mantzoros C. 20 years of leptin: role of leptin in human reproductive disorders Vol. 223, J Endocrinol. 2014;223(1):T49–62.
Farquhar J, Heiman M, Wong ACK, Wach R, Chessex P, Chanoine JP. Elevated umbilical cord ghrelin concentrations in small for gestational age neonates. J Clin Endocrinol Metab. 2003;88(9);4324–7. https://doi. org/10.1210/jc.2003-030265.
Zengul AG, Hoover SET, Chandler-Laney PC. Secondary analysis of gut hormone data from children with and without in utero exposure to gestational diabetes: Differences in the associations among ghrelin, GLP-1, and insulin secretion. Pediatr Obes. 2021;16(6): e12757. https://
Knopp RH, Warth MR, Charles D, Childs M, Li JR, Mabuchi H, et al. Lipoprotein metabolism in pregnancy, fat transport to the fetus, and the effects of diabetes. Biol Neonate. 1986;50(6):297–317. https://doi. org/10.1159/000242614.
Bucur-Grosu ML, Avasiloaiei A, Moscalu M, Dimitriu DC, Păduraru L, Stamatin M. Desacylated ghrelin and leptin in the cord blood of small-for-gestational-age newborns with intrauterine growth restriction. Acta Endocrinol (Copenh). 2019;15(3):305–310. aeb.2019.305.
Allbrand M, Åman J, Lodefalk M. Placental ghrelin and leptin expression and cord blood ghrelin, adiponectin, leptin, and C-peptide levels in severe maternal obesity. Journal of Maternal-Foetal and Neonatal Medicine. 2018;31(21): 2839–2846.