Comparative analysis of fatty acid composition in 84 accessions of flax (Linum usitatissimum L.)
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Department of Breeding and Agronomy of Fibrous Plants, Institute of Natural Fibres and Medicinal Plants, Poland
Laboratory of Genetics and Quality Breeding, Plant Breeding and Acclimatization Institute – National Research Institute, Poland
Corresponding author
Grażyna Silska   

Department of Breeding and Agronomy of Fibrous Plants , Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego71B, 60-630, Poznań, Poland
J Pre Clin Clin Res. 2019;13(3):118-129
Introduction and objective:
The aim of the study was to determine the content of five essential fatty acids in seed oil from 84 genotypes of flax (Linum usitatissimum L.) from the collection of genetic resources, stored at low temperatures of the long-term storage of the Plant Breeding and Acclimatization Institute in Radzików. The following fatty acids were evaluated: α-linolenic, linoleic, palmitic, stearic and oleic. The ranges of variability of fat content in flax seeds were also determined. In addition, it was calculated how many times the α-linolenic acid content is bigger than linoleic acid in seed oil of each accessions of flax.

Material and methods:
The research material consisted of 84 genotypes of common flax (Linum usitatissimum L.), collected and included in the samples. The fat content was determined by infrared analysis (calibration performed on the basis of a seed sample at IHAR-PIB in Poznań) by means of a NIRS 6500 spectrophotometer with a reflection detector within the range of 400–2500 nm.

The ranges of variability of individual fatty acids of 84 flax accessions are as follows: α-linolenic acid: 48.4% – 58.9%, linoleic acid: 10.3% – 17.3%, palmitic acid 4.2% – 6.6%, stearic acid 2.6% – 5.1% and oleic acid: 17.0% – 26.7%. The fat content in the seeds of the evaluated accessions ranged from 39.8% – 44.8%.

Flax seeds from the collection of the genetic resources are an excellent ingredient in food because they contain a large amount of α-linolenic acid. Dietary supplementation with linseed, also containing large amounts of α-linolenic acid and small amounts linoleic acid, allows provision of the body with the necessary ratio of the diunsaturated (n-6) to the triunsaturated fatty acid (n-3). In order to protect health, it is necessary to supplement the diet with a unique α-linolenic fatty acid that are present in only a few food ingredients.

The establishment of a field experiment and chemical analysis were financed from the long-term program “Plant improvement for sustainable agro-ecosystems, high-quality food and plant production for non-food purposes”.
Bhatty RS, Rowland. Measurement of alpha-linolenic acid in the development of edible oil flax. J Am Oil Chem Soc. 1990; 67: 364–67.
Burbulis N, Blinstrubiene A, Kupriene R. Effects of genotype and medium composition on linseed (Linum usitatissimum L.) ovary culture. Biologia. 2011; 66(3): 465–469.
Zając T, Klima K, Borowiec F, Witkowicz R, Barteczko J. Sets of herbs from the 18th and 20th centuries. Oilseed Crops 2002; 23: 275–86 (in Polish).
Ganorkar PM, Jain RK. Flaxes – a nutritional punch. Int Food Res J. 2013; 20(2): 519–55.
Wielebski F, Wójtowicz M, Spasibionek S. Drobnik J, Drobnik E. Effect of sowing density and habitat conditions on seed field, morphological plant charakter and field structure of yellow and Brown linseed cultivars (Linum usitatissimum L.). Fragm. Agron. 2016; 65: 124–33 (in Polish).
Oomah BD, Mazza G, Kenaschuk EO. Dehulling Characteristic of Flaxeed. Lebensm.- Wiss. u.-Technol. 1996; 29: 245–250.
Andruszewska A, Byczyńska M. Resistance of linseed cultivars from the Institute of Natural Fibres collection to Fusarium wilt. Oilseed Crops 2005; 26: 185–92 (in Polish).
Andruszewska A, Byczyńska M, Silska G. Healthiness of oil flax varieties from the seed collection considered as a row material for food industry. Prog Plant Prot. 2009; 49(1): 177–82 (in Polish).
Andruszczak S, Gawlik-Dziki U, Kraska P, Kwiecińska-Poppe E, Pałys E, Różyło K. Yield and quality traits of two linseed (Linum usitatissimum L.) cultivars as affected by some agronomic factors. Plant Soil Environ. 2015; 61(6): 247–252.
Drobnik J, Drobnik E. Sets of herbs from the 18th and 20th centuries. Farmacja Pol. 2009; 65: 348–55 (in Polish).
Bacelis K, Jankauskiene Z. Investigation and use of the Lithuanian flax genetic resources in the breeding programs. Environ Technology Resources. 2005; 85–92
Dabkevicius Z, Gelvonauskis B, Leistrumaite A. Investigation of genetic resources of cultivated plants in Lithuania. Biologija 2008; 54(2): 51–55.
Strączkowski M, Karczewska-Kupczewska M. Food in the prevention of civilization diseases (Selected topics). Proceedings of the 3st Congress of Agricultural Sciences “Science-Practice” (Scientific research in the process of shaping the Polish vision of the Common Agricultural Policyand the Common Fisheries Policy); 2015: 249–251 (Collective work edited by Chyłek EK and Pietras M) Sep 10; Warszawa. Poland: Bielsko Biała Pascal sp. z o.o. (in Polish).
Bartkowski L. Linseed – a natural source of health and beauty. Chemik. 2013; 67(3): 16–191.
Popa VM, Gruia A, Raba DN, Dumbrava D, Moldovan C, Bordean D, Mateescu C. Fatty acids composition and oil characteristics of linseed (Linum usitatissimum L.) from Romania. JAP&T. 2012; 18(2): 136–140.
Han H, Yilmaz H, Gulcin I. Antioxidant Activity of Flaxseed (Linum usitatissimm L.) shell and Analysis of Its Polyphenol Contents by L C-M S/M S. ACG publications. 2018; 12(4): 397–402.
Priyanka K, Sharma A, Sood DR. Flaxseed – a potential functional food source. J Food Sci Technol. 2015; 52(4): 1857–1871.
Silska G, Praczyk. Descriptors of characterization and evaluation of International Flax Database. Biul Inst Hod Rośl. 2013; 268: 161–171 (in Polish).
Walkowiak M, Silska G, Michalski K, Praczyk M. Characterization of a collection of linseed (Linum usitatissimum L.) with varying fat content and composition of fatty acids in seed oil. Proceedings of the 31st Polish Conference: Advances in genetics, breeding, technology and analytics of lipids; 2016 Feb 11–12; Poznań, Poland. content/uploads/2018/12/9910bc964f_1.2-Poz.15.
Silska G. Genetic resources of flax (Linum usitatissimum L.) as a very rich souce of α-linolenic acid. Herba Pol. 2017; 63: 26–33.
Byczyńska B, Krzymański J. A fast way to obtain fatty acid methyl esters for analysis by gas chromatography. Edible Fats. 1969; 13: 108–14 (in Polish).
PN-EN ISO 5508: 1996 Vegetable and animal oils and fats – Analysis of methyl esters of fatty acids by gas chromatography (in Polish).
PN-ISO 5509: 1996. Analysis of fatty acid methyl esters by gas chromatography (in Polish).
Pavelek M. Descriptors for the evaluation of flax. Workshop summary of the second meeting of the Flax Breeding Research Group of the European Cooperative Network on Flax; 1994 Nov 8–9; Brno, Czech Republic.
Nôžková J. Descriptor list for flax (Linum usitatissimum L.). 1 st ed. Nitra. Slovak University of Agriculture. 2011: 1–101.
Silska G, Praczyk M. Evaluation of the collection accessions of linseed (Linum usitatissimum L.). Oilseed Crops 2012; 33(1): 127‒38 (in Polish).
Silska G. The Unique Composition of Fatty Acids of Flax, from the Linum usitatissimum L. collection. Biomedical J SciTech Res. 2019; 18(4): 13731–13736.
Trziszka T. Nutraceuticals and their importance in human nutrition Proceedings of the 3st Congress of Agricultural Sciences “Science-Practice” (Scientific research in the process of shaping the Polish vision of the Common Agricultural Policy and the Common Fisheries Policy); 2015: 259–262 (Collective work edited by Chyłek EK and Pietras M) Sep 10; Warszawa. Poland: Bielsko Biała Pascal sp. z o.o. (in Polish).
WHO: Global strategy on diet, physical activity and health. 2004 http:// web.pdf.
Pieszka M, Pietras M. New directions in nutritional research – nutrigenomics. Roczniki Nauk Zootechnicznych. 2010; 37(2): 83–103 (in Polish).
Cichocka A. Type 2 diabetes. Part I. Epidemic of our time. Przemysł spożywczy 2013; 67: 38–40 (in Polish).
Silska G. The species (Linum usitatissimum L) in prevention of cancer diseases (in Poland). Herb plants, natural cosmetics and functional foods. Natural Med Oncol. 2017: 272–304.
Wierzejska R. Nutritional information on the packaging of food products – consumer approach. Przemysł spożywczy 201; 66: 43–45 (in Polish).
Bałasińska B, Jank M, Kulasek G. Properties and role of polyunsaturated fatty acids in maintaining human and animal health. Życie weterynaryjne. 2010; 85(9): 749–756 (in Polish).
Cichocka A. The recommended choice of food products in the prevention of cardiovascular disease. Proceedings of the 3st Congress of Agricultural Sciences “Science-Practice” (Scientific research in the process of shaping the Polish vision of the Common Agricultural Policy and the Common Fisheries Policy); 2015: 263–266 (Collective work edited by Chyłek EK and Pietras M) Sep 10; Warszawa. Poland: Bielsko Biała Pascal sp. z o.o. (in Polish).
Walczak Z, Starzycki M. Bromat. Evaluation of fatty acid profile of cold-pressed oils in the content of their recommended dietary intake by physically active people. Chem Toksykol. 2013; 3: 316–322.
Matławska I, Bylka W. Natural essential fatty acids in the prevention of civilization diseases. Herba Pol. 2007; 53(2): 39 (in Polish).
Simopoulos AP, The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed Pharmacotheraphy 2002; 56: 365–379.
Mińkowski K, Grześkiewicz S, Jerzewska M. Evaluation of the nutritional value of vegetable oils with a high content of linolenic acids based on the composition of fatty acids, tocopherols and sterols. Żywność. Nauka. Technologia. Jakość. 2011; 2(75), 124–135 (in Polish).
Rudzińska M, Wąsowicz E. Essential unsaturated fatty acids. In: Czapski J, Górecka D, editors. Pro-health food – ingredients and technology. 2015. p. 219–232 (in Polish).
Huerta-Yepez S, Tirado-Rodriguez AB, Hankinson O. Role of diets rich in omega-3 and omega-6 in the development of cancer. Bol Med Hosp Infant Mex. 2016; 73(6): 446–456. science/article/pii/S1665114616301423.
Burdge G. Alfa-linolenic acid metabolism in men and women: nutritional and biological implications. Curr Opin Clin Nutr Metab Care 2004; (7): 137–144.
Obiedzińska A, Waszkiewicz-Robak B, Cold pressed oils as functional foods. Żywność. Nauka. Technologia. Jakość. 2012; 1(80): 27–44 (in Polish).
Łoźna K, Kita A, Styczyńska M, Biernat J. The composition of fatty acids oils recommended in the prevention of civilization diseases. Probl Hig Epidemiol. 2012; 93(4): 871–875 (in Polish).
Decorti D, Tubaro F. Fatty acid composition and oxidation stability of hemp (Cannabis sativa L.) seed oil extracted by supercritical carbon dioxide. Industrial Crops and Products 2012; (36): 401–404.
Kluza-Wieloch M., Muśnicki C. Dynamics of qualitative chan ges of achenes in common sunflower (Helianthus annuus L.) during their ripening. Oilseed Crops 2006; (27): 231–242.
Silska G. The polish flax collection a source of seeds with therapeutic activity. Zagadnienia Doradztwa Rolniczego. 2016; (4): 73–81.
Jelińska M. Fatty acids – factors modifying cancer processes. Biuletyn Wydziału Farmaceutycznego AMW”, 2005 (1) (in Polish).
Wang YF, Jankauskiene Z, Qiu CS, Gruzdevienne E, Long SH, Alexopoulou Fiber Flax Breeding in China and Europe. J Natural Fibres. 2018; 15.
Razukas A, Jankauskiene Z, Jundulas J, Asakaviciute R. Research of technical crops (potato and flax) genetic resources in Lithuania. Agronomy Res. 2009; 7(1): 59–72.
Lorenc-Kukuła K, Amarowicz R, Oszmiański J, Doermann P, Starzycki M, Skała J, Żuk M, Kulma A, Szopa J. Pleiotropic Effect of Phenolic Compounds Content Increases in Transgenic Flax Plant. J Agric Food Chem. 2005; 53: 36853692.
Budwig J. Flax Oil as a True Aid against Arthrits, Heart Infarction and Cancer. 1994. Apple Publishing Co LTD.
Rodriguez-Leyva D, Basset C, McCullough R, Pierce G. The cardio- vascular effect of flaxseed and its omega-3 fatty acid, alpha-linolenic acid. Can J Cardiol. 2010; 26(9): 489–496.
Walkowiak M. The recombination breeding, mutagenesis and in vitro androgenesis in the investigation on linseed (Linum usitatissimum L.). Oilseed Crops 2007; 26: 151–157.
Green AG, Marshall DR. Isolation of induced mutants in linseed (Linum usitatissimum L.) having reduced linolenic acid content. Euphytica 1984; 33: 321–328.
Dribnenki JCP, Green AG. Linola TM 947 low linolenic acid flax. Can. J Plant Sci. 1995; 75: 201–202.
Bjelková M, Nôžková J, Fatrcová-Sramkova K, Tejklová E. Comparison of linseed (Linum usitatissimum L.) genotypes with respect to the content of polyunsaturated fatty acids. Chemical Papers 2012; 66(10): 972–976.
Bertrand M, Musa ÖM. Fatty Acid Composition, Tocopherol and Sterol Contents in Linseed (Linum usitatissimum L) Varieties. Iran J Chem Chem Eng. 2017; 36(3): 147–152.
Pali V, Mehta N. Evaluation of Oil Content and Fatty Acid Compositions of Flax (Linum usitatissimum L.) Varieties of India. J Agric Sci. 2014; 6(9): 198–207.
Silska G. Promotion of flax rich in bioactive compounds that protect health (Linum L.). Proceedings of Scientific Symposium “Gene resources of useful plants for breeding”. 2017 September 6–8; Kazimierz Dolny, Poland Bez-nazwy-23102017-070849.pdf.
Silska G. Seeds of common flax (Linum usitatissimum L.) as functional food in prevention of brain diseases (in Poland). Proceedings of the 5th International Conference: Herb plants, natural cosmetics and functional foods. New hopes for phytotherapy; 2018 May 10–11; Krosno, Poland. Abstrakt-Krosno-2018-1.pdf.
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