RESEARCH PAPER
Study on free amino acid concentrations in umbilical cord blood plasma of newborns from normoglycaemic and gestational diabetes-complicated pregnancies
 
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1
I Chair and Department of Oncological Gynaecology and Gynaecology, Medical University of Lublin, Poland
2
Chair and Department of Obstetrics and Pathology of Pregnancy, Medical University of Lublin, Poland
3
Department of Internal Diseases, Medical University of Lublin, Poland
CORRESPONDING AUTHOR
Agnieszka Grafka   

I Chair and Department of Oncological Gynaecology and Gynaecology, Medical University, Staszica 16, 20-081 Lublin, Poland
 
J Pre Clin Clin Res. 2016;10(1):12–22
KEYWORDS
ABSTRACT
Introduction and objective:
Gestational diabetes mellitus is a common disease among pregnant women. The aim of the study was to compare plasma concentrations of 24 amino acids in umbilical cord blood in newborns from normoglycaemic pregnancies versus those complicated by gestational diabetes. The relationship between birth weight and length of newborns and placental concentrations of individual amino acids were also assessed.

Material and Methods:
The study comprised 76 pregnant women at the gestational age of 37 weeks and more. The control group consisted of 31 women whose 75-g glucose load test excluded gestational diabetes mellitus. The study group encompassed 45 women with diagnosed gestational diabetes mellitus. The placental plasma concentrations of 24 amino acids were determined using ion-exchange chromatography with an automated amino acid analyser.

Results:
The concentrations of cysteic acid, aspartic acid, threonine, glutamic acid, cystine, and alpha-aminobutyric acid in umbilical cord blood plasma were higher in the group complicated by gestational diabetes compared to normoglycemic pregnancies; otherwise, concentrations of glutamine, alanine, valine, phenylalanine, lysine and, arginine were lower in the group complicated by gestational diabetes than in the normoglycaemic group.

Conclusions:
The results show that despite optimal control of carbohydrate metabolism during gestational diabetes mellitus, there are many abnormalities in the amino acids metabolism. This suggests that research on the effects of amino acids concentration in umbilical cord blood on the foetal development in gestational diabetes-complicated pregnancies should be continued.

 
REFERENCES (51)
1.
Polish Gyneacological Society Standards of Medical Care in management of women with diabetes. Ginekol Pol. 2011; 82(6): 474–479.
 
2.
Murthy EK, Pavlić-Renar I, Metelko Ž. Diabetes and pregnancy. Diabetol Croatica. 2002; 31–3: 131–146.
 
3.
Hawryluk J, Grafka A, Gęca T, Łopucki M. Gestational diabetes in the light of current literature. Pol Merkur Lek. 2015; 38(228): 344–347.
 
4.
Mahmud M, Mazza D. Preconception care of women with diabetes: a review of current guideline recommendations. BMC Women’s Health. 2010; 10(5): 1–7.
 
5.
Ulman-Włodarz I, Salomon-Perzyńska M. Cukrzyca ciążowa i nadciśnienie w ciąży – współczesne postępowanie. W: Zespół metaboliczny u kobiet – problem interdyscyplinarny (red.). Leszczyńska-Gorzelak B i Oleszczuk J. Oddział Lubelski Pol Tow Medycyny Perinatalnej. Lublin. 2010; 195–202 (in Polish).
 
6.
Avagliano L, Garò Ch, Marconi AM. Placental Amino Acids Transport in Intrauterine Growth Restriction. J Pregnancy. 2012; 972562: 1–6.
 
7.
Latendresse G, Founds S. The fascinating and complex role of the placenta in pregnancy and fetal well-being. J Midwifery Womens Health. 2015; 60(4): 360–370.
 
8.
Moore S, Spackman DH, Stein WH. Chromatography of amino acids on sulfonated polystyrene resins. An improved system. Anal Chem. 1958; 30(7): 1185–1190.
 
9.
Hyttinen V, Kaprio J, Kinnunen L, Kosekenvuo M, Tuomilehto J. Genetic liability of type 1 diabetes and the onset age among 22,650 young Finnish twin pairs: a nationwide follow-up study. Diabetes. 2003; 52(4): 1052–1055.
 
10.
Krentz AJ. Cukrzyca. Med Prakt. Kraków, 2001 (in Polish).
 
11.
Grissa O, Yessoufou A, Mrisak I, Hichami A, Amoussou-Guenou D, Grissa A, et al. Growth factor concentrations and their placental mRNA expression are modulated in gestational diabetes mellitus: possible interactions with macrosomia. BMC Pregnancy and Childbirth. 2010; 10:7. doi:10.1186/1471–2393–10–7.
 
12.
Kamaura M, Nishijima K, Takahashi M, Ando T, Mizushima S, Tochikubo O. Lifestyle modification in metabolic syndrome and associated changes in plasma amino acid profiles. Circ J. 2010; 74(11): 2434–2440.
 
13.
Tai ES, Tan ML, Stevens RD, Low YL, Muehlbauer MJ, Goh DL, et al. Insulin resistance is associated with a metabolic profile of altered protein metabolism in Chinese and Asian-Indian men. Diabetologia. 2010; 53(4): 757–767.
 
14.
Wender-Ożegowska E, Sporna M, Zawiejska A. Cukrzyca ciążowa (GDM) – patomechanizm i konsekwencje kliniczne. Terapia. 2007; 2: 24–30 (in Polish).
 
15.
Kinalski M, Śledziewski A, Majkowicz-Młynarczyk A, Kuźmicki M. Ciąża powikłana cukrzycą. Med Wieku Rozw. 2004; 8(3) część II: 691–701 (in Polish).
 
16.
Schaefer-Graf UM, Kjos SL, Kilavus O, Plagemann A, Brauer M, Dudenhausen JW, Vetter K. Determinants of fetal growth at different periods of pregnancies complicated by gestational diabetes mellitus or impaired glucose tolerance. Diabetes Care. 2003; 26(1): 193–198.
 
17.
Szymańska M, Bomba-Opoń DA, Celińska AM, Wielgoś M. Diagnostic of gestational diabetes mellitus and the prevalence of LGA (Large for Gestational Age). Ginekol Pol. 2007; 78(3): 177–181.
 
18.
Jędrzejowska E, Wilczyński J, Podciechowski L. Evaluation of the prognostic value of chosen maternal risk factors of complications existing among newborns of GDM mothers. Ginekol Pol. 2000; 70(10): 689–699.
 
19.
Zamłyński J, Olejek A, Więcek A, Mańka G, Chudek J, Bodzek P, Damasiewicz-Bodzek A. The infl uence of metabolic changes on intrauterine fetal growth in normal gestation and gestation complicated by diabetes. Postępy Hig Med Dośw. 2005; 59: 490–495.
 
20.
Hod M, Yogev Y. Goals of metabolic management of gestational diabetes: is it all about the sugar? Diabetes Care. 2007; 30Suppl2: S180-S187.
 
21.
Sertkaya AC, Kafkasli A, Turkcuoglu I, Karabulut AB. Asymmetric dimethylarginine level in hyperglycemic gestation. Endocrine. 2011; 40(2): 237–242.
 
22.
Hay WW. Placental-fetal glucose exchange and fetal glucose metabolism. Trans Am Clin Climatol Assoc. 2006; 117: 321–340.
 
23.
Butte NF, Hsu HW, Thotathuchery M, Wong WW, Khoury J, Reeds P. Protein Metabolism in Insulin-Treated Gestational Diabetes. Diabetes Care. 1999; 22(5): 806–811.
 
24.
Stenninger E, Achollin J, Aman J. Neonatal macrosomia and hypoglycaemia in children of mothers with insulin-treated gestational diabetes mellitus. Acta Paediatr Scand. 1991; 80(11): 1014–1018.
 
25.
Metzger BE. Biphasic effects of maternal metabolism on fetal growth: quintessential expression of fuel-mediated teratogenesis. Diabetes. 1991; 40(Suppl2): 99–105.
 
26.
Kinalski M, Śledziewski A, Telejko B, Kowalska I, Kretowski A, Zarzycki W, Kinalska I. Lipid peroxidation, antioxidant defence and acid-base status in cord blood at birth: the influence of diabetes. Horm Metab Res. 2001; 33(4): 227–231.
 
27.
McClain PE, Metcoff J, Crosby WM, Costiloe JP. Relationship of maternal amino acid profiles at 25 weeks of gestation to fetal growth. Am J Clin Nutr. 1978; 31(3): 401–407.
 
28.
Cetin I, de Santis MS, Taricco E, Radaelli T, Teng C, Ronzoni S, et al. Maternal and fetal amino acid concentrations in normal pregnancies and in pregnancies with gestational diabetes mellitus. Am J Obstet Gynecol. 2005; 192(2): 610–617.
 
29.
Catalano PM, Tyzbir ED, Wolfe RR, Roman NM, Amini SB, Sims EA. Longitudinal changes in basal hepatic glucose production and suppression during insulin infusion in normal pregnant women. Am J Obstet Gynecol. 1992; 167(4Pt1): 913–919.
 
30.
Shao J, Catalano PM, Yamashita H, Ruyter I, Smith S, Youngren J, Friedman JE. Decreased insulin receptor tyrosine kinase activity and plasma cell membrane glycoprotein-1 overexpression in skeletal muscle from obese women with gestational diabetes mellitus (GDM): evidence for increased serine/threonine phosphorylation in pregnancy and GDM. Diabetes. 2000; 49(4): 603–610.
 
31.
Cariappa R, Heath-Monnig E, Smith CH. Isoforms of amino acid transporters in placental syncytiotrophoblast: plasma membrane localization and potential role in maternal/fetal transport. Placenta. 2003; 24(7): 713–726.
 
32.
Józwik M, Teng C, Wilkening RB, Meschia G, Battaglia FC. Reciprocal inhibition of umbilical uptake within groups of amino acids. Am J Physiol Endocrinol Metab. 2004; 286(3): E376-E383.
 
33.
Constancia M, Hemberger M, Hughes J, Dean W, Ferguson-Smith A, Fundele R, et al. Placental-specific IGF-II is a major modulator of placental and fetal growth. Nature. 2002; 417(6892): 945–948.
 
34.
Jansson N, Greenwood SL, Johansson BR, Powell TL, Jansson T. Leptin stimulates the activity of the system A amino acid transporter in human placental villous fragments. J Clin Endocrinol Metab. 2003; 88(3): 1205–1211.
 
35.
Pappa KI, Vlachos G, Theodora M, Roubelakj M, Angelidou K, Antsaklis A. Intermediate metabolism in association with the amino acid profile during the third trimester of normal pregnancy and diet-controlled gestational diabetes. Am J Obstet Gynecol. 2007; 196(1): 65.e1–5.
 
36.
Freyse EJ, Fischer U, Albrecht G, Salzsieder E. Alterations in alanine metabolism in diabetic dogs during short-term treatment with an artificial B cell. Diabetologia. 1985; 28(10): 763–768.
 
37.
Meynial-Denis D, Chavaroux A, Foucat L, Mignon M, Prugnaud J, Bayle G, et al. Contribution of proteolysis and de novo synthesis to alanine production in diabetic rat skeletal muscle: a 15N/1H nuclear magnetic resonance study. Diabetologia. 1997; 40(10): 1159–1165.
 
38.
Porcellati F, Pampanelli S, Rossetti P, Busciantella Ricci N, Marzotti S, Lucidi P, et al. Effect of the amino acid alanine on glucagon secretion in non-diabetic and type 1 diabetic subjects during hyperinsulinaemic euglycaemia, hypoglycaemia and post-hypoglycaemic hyperglycaemia. Diabetologia. 2007; 50(2): 422–430.
 
39.
Lee CC, Watkins SM, Lorenzo C, Wagenknecht LE, Il’vasova D, Chen YI, et al. Branched-Chain Amino Acids and Insulin Metabolism: The Insulin Resistance Atherosclerosis Study (IRAS). Diabetes Care. 2016; Eepub ahead of print.
 
40.
Reece EA, Coustan DR, Sherwin RS, Tuck S, Bates S, O’Connor T, Tamborlane WV. Does intensive glycemic control in diabetic pregnancies result in normalization of other metabolic fuels? Am J Obstet Gynecol. 1991; 165(1): 126–130.
 
41.
Jóźwik M, Teng C, Wilkening RB, Meschia G, Tooze J, Chung M, Battaglia FC. Effects of branched-chain amino acids on placental amino acid transfer and insulin and glucagon release in the ovine fetus. Am J Obstet Gynecol. 2001; 185(2): 487–495.
 
42.
Garcia RF, Gazola VA, Barrena HC, Hartmann EM, Berti J, Toyama MH, et al. Blood amino acids concentration during insulin induced hypoglycemia in rats: the role of alanine and glutamine in glucose recovery. Amino Acids, 2007; 33(1): 151–155.
 
43.
Reimann F, Williams L, da Silva Xavier G, Rutter GA, Gribble FM. Glutamine potently stimulates glucagon-like peptide-1 secretion from GLUTag cells. Diabetologia. 2004; 47(9): 1592–1601.
 
44.
Franconi F, Loizzo A, Ghirlanda G, Seghieri G. Taurine supplementation and diabetes mellitus. Curr Opin Clin Nutr Metab Care. 2006; 9(1): 32–36.
 
45.
Camelo Jr JS, Martinez FE, Gonçalves AL, Monteiro JP, Jorge SM. Plasma amino acids in pregnancy, placental intervillous space and preterm newborn infants. Braz J Med Biol Res. 2007; 40(7): 971–977.
 
46.
Hay WW Jr. Placental transport of nutrients to the fetus. Horm Res. 1994; 42(4–5): 215–222.
 
47.
McBride KL, Belmont JW, O`Brien WE, Amin TJ, Carter S, Lee BH. Heritability of plasma amino acid levels in different nutritional states. Mol Genet Metab. 2007; 90(2): 217–220.
 
48.
Shikata N, Maki Y, Noguchi Y, Moru M, Hanai T, Takahashi M, Okamoto M. Multi-layered network structure of amino acid (AA) metabolism characterized by each essential AA-deficient condition. Amino Acids. 2007; 33(1): 113–121.
 
49.
Haase TN, Rasmussen M, Jaksch CA, Gaarn LW, Petersen CK, Billestrup N, Nielsen JH. Growth arrest specific protein (GAS) 6: a role in the regulation of proliferation and functional capacity of the perinatal rat beta cell. Diabetologia. 2013; 56(4): 763–773.
 
50.
Duggleby SL, Jackson AA. Protein amino acid and nitrogen metabolism during pregnancy: how might the mother meet the needs of her fetus? Curr Opin Clin Nutr Metab Care. 2002; 5(5): 503–509.
 
51.
Robinson S, Prendergast CH. Protein metabolism in pregnancy. Baillieres Clin Endocrinol Metab. 1996; 10(4): 571–587.
 
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