Effect of dietary fat type on plasma lipid profile and leptin concentration in rats fed on high-sucrose diets
More details
Hide details
Division of Nutrition Physiology, Department of Dietetics, Faculty of Human Nutrition and Consumer Science, Warsaw University of Life Sciences – SGGW, Warsaw, Poland
Agata Krawczyńska   

Division of Nutrition Physiology, Department of Dietetics, Faculty of Human Nutrition and Consumer Science, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159c, 02-787 Warsaw, Poland.
J Pre Clin Clin Res. 2010;4(1):57–62
The aim of the study was to investigate the effect of different dietary fat type on plasma leptin concentration and lipid profile in male Wistar rats fed normo-fat, normo-protein, high-sucrose (5, 19 and 35 % w/w, respectively) diets. The experiment was conducted on 21 adult male rats (260 ± 20g) fed diets with different fat sources: lard (L), grapeseed oil (G) and fl axseed oil (F). Radioimmunoassay was used to measure leptin concentration and enzymaticcolorimetric methods to estimate lipid profile. Total cholesterol (TC), high density lipoprotein cholesterol (HDL) and triglycerides (TG) plasma concentrations were higher in group L and G than F (ANOVA p≤0,01; p≤0,05 and p≤0,005, respectively), whereas low density lipoprotein cholesterol (LDL) level was higher in group L than G and F (ANOVA p≤0,05). Leptin concentration was signifi cantly higher in group L in comparison to F (ANOVA p≤0,04). Significant positive correlations were found between plasma leptin concentration and final body weight, TC, HDL and TG (r = 0,64, p≤0,006; r=0,72, p≤0,002; r=0,69, p≤0,003; r=0,86, p≤0,00004 respectively). It can be observed that flaxseed oil rich in n-3 polyunsaturated fatty acids (PUFA) profi tably influenced not only lipid profile lowering its parameters but also reduced leptin concentration which can suggest approximate lipidogenic potential of both grapeseed oil (rich in PUFA n-6) and lard (rich in monounsaturated and saturated fatty acids). The results provided evidence that dietary fat type can influence cardiovascular disease risk parameters when high-sucrose diet is consumed.
Zhang Y, Proenca R, Maff ei M, Barone M, Leopold L, Friedman JM: Positional cloning of the mouse obese gene and its human homologue. Nature 1994, 372, 425-432.
Anubhuti, Arora S: Leptin and its metabolic interactions: an update. Diabetes Obes Metab 2008, 10(11), 973-993.
Beltowski L: Leptin and atherosclerosis. Atherosclerosis 2006, 189, 47-60.
Ren J: Leptin and hyperleptinemia - from friend to foe for cardiovascular function. J Endocrinol 2004, 181, 1-10.
Zhang Y, Scarpace PJ: The role of leptin in leptin resistance and obesity. Physiol Behav 2006, 88, 249-256.
Orr J, Davy B: Dietary infl uences on peripheral hormones regulating energy intake: potential applications for weight management. J Am Diet Assoc 2005, 105(7), 1115-1124.
Mizuno TM, Bergen H, Funabashi T, Kleopoulos SP, Zhong YG, Bauman WA, Mobbs CV: Obese gene expression: reduction by fasting and stimulation by insulin and glucose in lean mice, and persistent elevation in acquired (diet-induced) and genetic (yellow agouti) obesity. Proc Natl Acad Sci USA 1996, 93, 3434 -3438.
Fernandez-Quintela A, Churruca I, Portillo MP: The role of dietary fat in adipose tissue metabolism. Public Health Nutr 2008, 10, 1126- 1131.
Chong MF, Fielding BA, Frayn KN: Metabolic interaction of dietary sugars and plasma lipids with a focus on mechanisms and de novo lipogenesis. Proc Nutr Soc 2007, 66, 52-59.
Poli A. Marangoni F, Paoletti R, Mannarino E, Lupattelli G, Notarbartolo A, Aureli P, Bernini F, Cicero A, Gaddi A, Catapano A, Cricelli C, Gattone M, Marrocco W, Porrini M., Stella R., Vanotti A, Volpe M, Volpe R, Cannella C, Pinto A, Del Toma E, La Vecchia C, Tavani A, Manzato E, Riccardi G, Sirtori C, Zambon A; Nutrition Foundation of Italy: Non-pharmacological control of plasma cholesterol levels. Nutr Metab Cardiovasc Dis 2008, 18, S1-S16.
Calder PC: n-3 Fatty acids and cardiovascular disease: evidence explained and mechanisms explored. Clin Sci 2004, 107, 1-11.
Massaro M, Scoditti E, Carluccio MA, Montinari MR, De Caterina R: Omega-3 fatty acids, infl ammation and angiogenesis: nutrigenomic eff ects as an explanation for anti-atherogenic and anti-infl amatory eff ects of fi sh and fi sh oils. J Nutrigenet Nutrigenomics 2008, 1, 4-23.
Lopez D, Moller M, Denicola A, Casos K, Rubbo IL, Ruiz-Sanz,JL, Mitjavila MT. Long-chain n-3 polyunsaturated fatty acid from fi sh oil modulates aortic nitric oxide and tocopherol status in the rat. Br J Nutr 2008, 17, 1-9.
Gately S, Li WW: Multiple roles of COX-2 in tumor angiogenesis: a target for antiangiogenic therapy. Semin Oncol 2004, 31, 2-11.
Willett WC: The role of dietary n-6 fatty acids in the prevention of cardiovascular disease. J Cardiovasc Med 2007, 8 Suppl 1, S42-45.
Merat S, Casanada F, Sutphin M, Palinski W, Reaven PD: Western-type diets induce insulin resistance and hyperinsulinemia in LDL receptordefi cient mice but do not increase aortic atherosclerosis compared with.Normoinsulinemic Mice in Which Similar Plasma Cholesterol Levels Are Achieved by a Fructose-Rich Diet', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 19, no. 5, pp. 1223-1230