RESEARCH PAPER
Conjugated linoleic acid has no effects on atherosclerosis but induces liver steatosis in apoE/LDLR -/- mice fed a fructose-rich diet
 
More details
Hide details
1
Department of Human Nutrition, Faculty of Food Technology, Agricultural University, Cracow, Poland
2
Department of Histology, Jagiellonian University Medical College, Cracow, Poland
3
Department of Experimental Pharmacology, Jagiellonian University Medical College, Cracow, Poland
CORRESPONDING AUTHOR
Renata B. Kostogrys
Department of Human Nutrition, Faculty of Food Technology, Agricultural University, Balicka 122, 30-149 Cracow, Poland.
 
J Pre Clin Clin Res. 2010;4(2):118–121
KEYWORDS
ABSTRACT
The current study was designed to investigate the effects of CLA on several known risk factors of atherosclerosis in apoE/LDLR-/- mice fed fructose diet. The mice at the age of 2 months were divided into 2 experimental groups and fed the following diets: I- AIN-93G + 60% fructose (Fructose) and II- AIN-93G + 60% fructose + 0.5% CLA (Fructose+CLA). After 2 months of feeding, plasma lipid profile, glucose concentration, liver fatty acid composition, and quantification of atherosclerosis were analyzed. There were no differences in body and kidney weight. Liver weight was significantly increased in the CLA-treatment group which was related to higher liver lipid concentration. No changes in plasma lipid profile nor liver fatty acid composition were observed between experimental groups. These results indicate that CLA increased liver lipid accumulation. It may cause liver steatosis in experimental mice.
 
REFERENCES (32)
1.
Howard BV, Wylie-Rosett J: Sugar and cardiovascular disease: A statement for healthcare professionals from the Committee on Nutrition of the Council on Nutrition, Physical Activity, and Metabolism of the American Heart Association. Circulation 2002, 106(4), 523-527.
 
2.
Kennedy A, Martinem K, Schmidt S, Mandrup S, LaPoint K, McIntosh M: Antiobesity mechanisms of action of conjugated linoleic acid. J Nutr Biochem 2010, 3, 171 179.
 
3.
Belury MA: Inhibition of carcinogenesis by conjugated linoleic acid: potential mechanisms of action. J Nutr 2002, 132, 2995-2998.
 
4.
Field CJ, Schley PD: Evidence for potential mechanisms for the effect of conjugated linoleic acid on tumor metabolism and immune function: lessons from n-3 fatty acids. Am J Clin Nutr 2004, 79, 1190S-1198S.
 
5.
Cho HJ, Kim EJ, Lim SS, Kim MK, Sung MK, Kim JS, Park JH: Trans- 10,cis-12, not cis-9,trans-11, conjugated linoleic acid inhibits G1-S progression in HT-29 human colon cancer cells. J Nutr 2006, 136, 893–898.
 
6.
Noone EJ, Roche HM, Nugent AP, Gibney MJ: The effect of dietary supplementation using isomeric blends of conjugated linoleic acid on lipid metabolism in healthy human subjects. Brit J Nutr 2002, 88, 243-251.
 
7.
Tricon S, Burdge GC, Kew S, Banerjee T, Russell JJ, Jones EL, Grimble RF, Williams CM, Yaqoob P, Calder PC: Opposing effects of cis-9,trans- 11 and trans-10,cis-12 conjugated linoleic acid on blood lipids in healthy humans. Am J Clin Nutr 2004, 80, 614-620.
 
8.
Valeille K, Gripois D, Blouquit MF, Souidi M, Riottot M, Bouthegourd JC, Serougne C, Martin JC: Lipid atherogenic risk markers can be more favourably influenced by the cis-9 trans-11-octadecadienoate isomer than a conjugated linoleic acid mixture or fish oil in hamsters. Brit J Nutr 2004, 91, 191-199.
 
9.
Arbones-Mainar JM, Navarro MA, Acin S, Guzman MA, Arnal C, Surra JC, Carnicer R, Roche HM, Osada J: Trans-10, cis-12- and cis-9, trans-11-Conjugated Linoleic Acid Isomers Selectively Modify HDLApolipoprotein Composition in Apolipoprotein E Knockout Mice. J Nutr 2006, 136, 353-359.
 
10.
Ryder JW, Portocarrero CP, Song XM, Cui L, Yu M, Combatsiaris T, Galuska D, Bauman DE, Barbano DM, Charron MJ, Zierath JR, Houseknecht KL: Isomer-specific antidiabetic properties of conjugated linoleic acid - Improved glucose tolerance, skeletal muscle insulin action, and UCP-2 gene expression. Diabetes 2001, 50, 1149 1157.
 
11.
Noto A, Zahradka P, Ryz NR, Yurkova N, Xie XP, Taylor CG: Dietary conjugated linoleic acid preserves pancreatic function and reduces inflammatory markers in obese, insulin-resistant rats. Metabolism 2007, 56(1), 142-151.
 
12.
Mersmann H.J: Mechanism for conjugated linoleic acid-mediated reduction In fat deposition. J Anim Sci 2002, 80(E. Suppl. 2), E126- E134.
 
13.
Evans M, Brown J, McIntosh M: Isomer-specific effects of conjugated linoleic acid (CLA) on adiposity and lipid metabolism. J Nutr Biochem 2002, 13, 508-516.
 
14.
Terpstra AH, Javadi M, Beynen AC, Kocsis S, Lankhorst AE, Lemmens AG, Mohede IC: Dietary conjugated linoleic acids as free fatty acids and triacylglycerols similarly affect body composition and energy balance in mice. J Nutr 2003, 133, 3181-3186.
 
15.
Wang Y, Jones PJH: The role of conjugated linoleic acid in human health. Dietary conjugated linoleic acid and body composition. American J Clin Nutr 2004, 79, 1153S-1158S.
 
16.
Purushotham A, Shrode G, Wendel A, Liu L, Belury M: Conjugated linoleic acid does not reduce body fat but decreases hepatic steatosis in adult Wistar rats. J Nutr Biochem 2007, 18(10), 676-684.
 
17.
Reeves PG, Nielsen FH, Fahey GC: AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 1993, 123(11), 1939-1951.
 
18.
Nicoletti A, Kaveri S, Caligiuri G, Bariety J, Hansson GK: Immunoglobulin treatment reduces atherosclerosis in apo E knockout mice. J Clin Invest 1998, 102, 910-918.
 
19.
Elhage R, Jawien J, Rudling M, Ljunggren HG, Takeda KS, Akira S, Bayard F, Hansson GK: Reduced atherosclerosis in interleukin-18 deficient apolipoprotein E – knockout mice. Cardiovasc Res 2003, 59, 234-240.
 
20.
Robertson AK, Rudling M, Zhou X, Gorelik L, Flavell RA, Hansson GK: Disruption of TGF-beta signaling in T cells accelerates atherosclerosis. J Clinical Invest 2003, 112, 1342-1350.
 
21.
Belury MA, Kempa-Steczko A: Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids 1997, 32, 199-204.
 
22.
DeLany JP, Blohm F, Truett AA, Scimeca JA, West DB: Conjugated linoleic acid rapidly reduces body fat content in mice without affecting energy intake. Am J Physiol 1999, 276, 1172-1179.
 
23.
Javadi M, Beynen AC, Hovenier R, Lankhorst A, Lemmens AG, Terpstra AH, Geelen MJ: Prolonged feeding of mice with conjugated linoleic acid increases hepatic fatty acid synthesis relative to oxidation. J Nutr Biochem 2004, 15(11), 680-687.
 
24.
Munday JS, Thompson KG, James KA: Dietary conjugated linoleic acids promote fatty streak formation in the C57BL/6 mouse atherosclerosis model. Brit J Nutr 1999, 81, 251-255.
 
25.
Kritchevsky D, Tepper SA, Wright S, Tso P, Czarnecki SK: Influence of conjugated linoleic acid (CLA) on establishment and progression of atherosclerosis in rabbits. J Am Coll Nutr 2000, 19, 472S-477S.
 
26.
Kritchevsky D, Tepper SA, Wright S, Czarnecki SK: Influence of graded levels of conjugated linoleic acid (CLA) on experimental atherosclerosis in rabbits. Nutr Res 2002, 22, 1275-1279.
 
27.
Wilson TA, Nicolosi RJ, Chrysam M, Kritchevsky D: Conjugated linoleic acid reduces early aortic atherosclerosis greater than linoleic acid in hypercholesterolemic hamsters. Nutr Res 2000, 20, 1795-1805.
 
28.
Arbones-Mainar JM, Navarro MA, Guzman MA, Arnal C, Surra JC, Acin S, Carnicer R, Osada J, Roche HM: Selective effect of conjugated linoleic acid isomers on atherosclerotic lesion development in apolipoprotein E knockout mice. Atherosclerosis 2006, 189(2), 318-327.
 
29.
De Roos B, Rucklidge G, Reid M, Pickard K, Navarro MA, Arbone´s- Mainar JM, Guzman-Garcia MA, Osada J, Browne J, Loscher CE, Roche HM: Divergent mechanisms of cis9, trans11- and trans10, cis12- conjugated linoleic acid affecting insulin resistance and inflammation in apolipoprotein E knockout mice: a proteomics approach. FASEB J 2005, 19, 1746-1748.
 
30.
Valeille K, Ferezou J, Amsler G, Quignard-Boulange A, Parquet M, Gripois D, Dorovska-Taran V, Martin JC: A cis-9,trans-11-conjugated linoleic acid-rich oil reduces the outcome of atherogenic process in hyperlipidemic hamster. Am J Physiol-Heart C 2005, 289(2), H652- H659.
 
31.
Toomey S, Roche H, Fitzgerald D, Belton O: Regression of preestablished atherosclerosis in the apoE(-/-) mouse by conjugated linoleic acid. Biochem Soc T 2003, 31, 1075-1079.
 
32.
Arbones-Mainar JM, Navarro MA, Acin S, Guzman MA, Arnal C, Surra JC, Carnicer R, Roche HM, Osada J. Trans-10, cis-12-and cis-9, trans-11-conjugated linoleic acid isomers selectively modify HDLapolipoprotein composition in apolipoprotein E knockout mice. J Nutr 2006, 136(2), 353-359.
 
eISSN:1898-7516
ISSN:1898-2395