RESEARCH PAPER
Circulating cytokeratin-18 and tumour necrosis factor-α in patients with alcoholic liver cirrhosis
 
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1
Department of Internal Diseases, Medical University of Lublin, Poland
2
Department of Family Medicine, Medical University of Lublin, Poland
3
Individual Medical Practice, Lublin, Poland
4
Department of Mathematics and Medical Biostatistics, Medical University of Lublin, Poland
5
Department of Medical Chemistry, Medical University of Lublin
6
Department of Trauma Surgery and Emergency Medicine, Medical University of Lublin, Poland
7
Department of Ethics and Human Philosophy, Medical University of Lublin, Poland
CORRESPONDING AUTHOR
Paweł Kiciński   

Department of Family Medicine, Medical University, Staszica 11, 20-081 Lublin, Poland
 
J Pre Clin Clin Res. 2016;10(2):87–90
KEYWORDS
ABSTRACT
Introduction:
The aim of the study was to assess the usefulness of TNF-α and CK-18 as diagnostic markers of alcoholic liver cirrhosis. Additionally, the effects of the stage of liver cirrhosis on concentrations of TNF-α and CK-18, as well as their correlation, were evaluated.

Material and Methods:
Sixty-two patients with alcoholic liver cirrhosis treated in various hospitals were randomly enrolled. The stage of cirrhosis was assessed according to the Child-Turcotte-Pugh scoring system. The control group consisted of 31 healthy people without liver disease. Concentrations of TNF-α and cytokeratin-18 in blood plasma of patients and controls were measured using the sandwich enzyme immunoassay technique, with commercially available quantitative ELISA test kits

Results:
The concentration of CK-18 was statistically higher in patients with alcoholic liver cirrhosis, compared to the control group. The concentration of TNF-α was significantly higher in patients with alcoholic liver cirrhosis, compared to the control group. Higher concentrations of TNF-α were found only in patients with stage C and B alcoholic liver cirrhosis, compared to healthy persons.

Conclusions:
The levels of TNF-α and total CK-18 were higher in patients with alcoholic liver cirrhosis than in healthy individuals. No correlation was found between the level of CK-18 and stage of liver cirrhosis.

 
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