Immobilization and experimental periodontitis in tibial anterior muscle of wistar rats
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Universidade Estadual do Oeste do Paraná, Brazil
Corresponding author
Gladson Ricardo Flor Bertolini   

Universidade Estadual do Oeste do Paraná, Rua Universitária, 2069., 85819110, Cascavel, Brazil
J Pre Clin Clin Res. 2019;13(1):1-4
Introduction and objective:
Periodontal disease (PD) is a chronic inflammation, locally attacking the supporting tissues of the tooth; this inflammatory condition can affect other systems, such as muscle tissue, which is also susceptible to several types of injury such as caused by immobilization. The aim of this study was to assess whether periodontal disease potentiates the deleterious effects of immobilization of the anterior tibial muscle of Wistar rats.

Material and methods:
Twenty animals were randomly assigned to four groups (n=5): Control Group (CG), Periodontal Disease Group (PDG), Immobilized Group (IG) and PD plus Immobilization Group (PDIG). PD was induced on day 1 of the experiment by placing a cotton thread in the first lower molars for 30 days, favouring the accumulation of bacterial plaque. On day 15, the right pelvic limb was immobilized for 15 days. On day 30, the animals were euthanized and the tibialis anterior muscle was collected with further processing for histomorphometric analysis.

. In the cross-sectional area there were PDIG differences between CG and PDG. For the larger diameter, CG was higher than IG and PDIG. For the smaller diameter, PDIG presented a lower value among all the groups in the same way with the number of nuclei. For connective tissue, there was an increase in the amount between the CG and PDIG, IG and PDIG groups, as well as PDG and PDIG.

Periodontal disease potentiated the deleterious effects of immobilization on the anterior tibialis muscle.

Toledo GB, Cruz I. The importance of oral hygiene in Intensive Care Unit as a way of prevention of nosocomial infection – literature review. J Spec Nurs Care [Internet]. 2009;2(1). Available from: http://www.
Ramos MMB, Mendonça MR de, Pellizzer EP, Okamoto AC, Júnior EGJ. Associação entre a doença periodontal e doenças sistêmicas crônicas – revisão de literatura / Association between periodontal disease and chronic systemic disease – literature review. Arch Heal Invest. 2013;2(1): 24–31.
Fernandez-Solari J, Barrionuevo P, Mastronardi CA. Periodontal disease and its systemic associated diseases. Mediators Inflamm. 2015; 2015: 153074.
Darveau RP, Tanner A, Page RC. The microbial challenge in periodontitis. Periodontol 2000. 1997; 14(79): 12–32.
Leite MA, de Mattia TM, Kakihata CMM, Bortolini BM, de Carli Rodrigues PH, Bertolini GRF, et al. Experimental periodontitis in the potentialization of the effects of immobilism in the skeletal striated muscle. Inflammation. 2017; 40(6): 2000–11.
França EÉT, Ferrari F, Fernandes P, Cavalcanti R, Duarte A, Martinez BP, et al. Physical therapy in critically ill adult patients: Recommendations from the Brazilian Association of Intensive Care Medicine Department of Physical Therapy. Rev Bras Ter Intensiva. 2012; 24(1): 6–22.
Ochala J, Gustafson AM, Diez ML, Renaud G, Li M, Aare S, et al. Preferential skeletal muscle myosin loss in response to mechanical silencing in a novel rat intensive care unit model: Underlying mechanisms. J Physiol. 2011; 589(8): 2007–26.
Jackman RW, Kandarian SC. The molecular basis of skeletal muscle atrophy. AJP Cell Physiol. 2004; 287(4): C834–43.
Piovesan RF, Fernandes KPS, Alves AN, Teixeira VP, Silva Junior JA, Martins MD, et al. Effect of nandrolone decanoate on skeletal muscle repair. Int J Sports Med. 2013; 34(1): 87–92.
Carvalho MM, Martins WR, Blackzick JC, Cruz Júnior CA da, Souza HA de, Kückelhaus SAS, et al. Análise morfométrica dos efeitos da imobilização sobre o músculo gastrocnêmio de ratos. Univ Ciências da Saúde. 2013; 11(2): 99–106.
Carvalho CMM de, Shimano AC, Volpon JB. Efeitos da imobilização e do exercício físico em algumas propriedades mecânicas do músculo esquelético. Rev Bras Eng Biomédica. 2002; 18(2): 65–73.
Ramírez C, Russo TL, Delfino G, Peviani SM, Alcântara C, Salvini TF. Effect of tibiotarsal joint inflammation on gene expression and cross-sectional area in rat soleus muscle. Brazilian J Phys Ther. 2013; 17(3): 244–54.
Ramírez C, Russo T, Sandoval M, Abbade Dentillo A, Abreu Silca Couto M, Quagliotti Durigan J, et al. Joint inflammation alters gene and protein expression and leads to atrophy in the tibialis anterior muscle in rats. Am J Phys Med Rehabil. 2011; 90(11): 930–9.
Winkelman C. Inactivity and inflammation: selected cytokines as biologic mediators in muscle dysfunction during critical illness. AACN Clin Issues. 2004; 15(1): 74–82.
Nassar PO, Nassar CA, Guimarães MR, Aquino SG, Andia DC, Muscara MN, et al. Simvastatin therapy in cyclosporine A-induced alveolar bone loss in rats. J Periodontal Res. 2009; 44(4): 479–88.
Kunz RI, Coradini JG, Silva LI, Bertolini GRF, Brancalhão RMC, Ribeiro LFC. Effects of immobilization and remobilization on the ankle joint in Wistar rats. Brazilian J Med Biol Res. 2014; 47(10): 842–9.
Mallinson JE, Murton AJ. Mechanisms responsible for disuse muscle atrophy: Potential role of protein provision and exercise as countermeasures. Nutrition. 2013; 29(1): 22–8.
Slimani L, Vazeille E, Deval C, Meunier B, Polge C, Dardevet D, et al. The delayed recovery of the remobilized rat tibialis anterior muscle reflects a defect in proliferative and terminal differentiation that impairs early regenerative processes. J Cachexia Sarcopenia Muscle. 2015; 6(1): 73–83.
Fujita N, Murakami S, Arakawa T, Miki A, Fujino H. The combined effect of electrical stimulation and resistance isometric contraction on muscle atrophy in rat tibialis anterior muscle. Bosn J Basic Med Sci. 2011; 11(2): 74–9.
Bodine SC. Disuse-induced muscle wasting. Int J Biochem Cell Biol. 2013; 45(10): 2200–8.
Brooks NE, Myburgh KH. Skeletal muscle wasting with disuse atrophy is multi-dimensional: The response and interaction of myonuclei, satellite cells and signaling pathways. Front Physiol. 2014; 5(99): 10.3389.
Bodine SC, Baehr LM. Skeletal muscle atrophy and the E3 ubiquitin ligases MuRF1 and MAFbx/atrogin-1. Am J Physiol Endocrinol Metab. 2014; 307(6): E469–84.
Pellegrino MA, Desaphy JF, Brocca L, Pierno S, Camerino DC, Bottinelli R. Redox homeostasis, oxidative stress and disuse muscle atrophy. J Physiol. 2011; 589(9): 2147–60.
Oliveira JAP, Hoppe CB, Gomes MS, Grecca FS, Haas AN. Periodontal disease as a risk indicator for poor physical fitness: A cross-sectional observational study. J Periodontol. 2015; 86(1): 44–52.
Fink H, Helming M, Unterbuchner C, Lenz A, Neff F, Martyn JAJ, et al. Systemic inflammatory response syndrome increases immobilityinduced neuromuscular weakness. Crit Care Med. 2008; 36(3): 910–6.
Hasturk H, Kantarci A, Van Dyke TE. Oral inflammatory diseases and systemic inflammation: Role of the macrophage. Front Immunol. 2012; 3(118): 10.3389.
Taylor JJ. Protein biomarkers of periodontitis in saliva. ISRN Inflamm. 2014; 2014: 593151.
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