Introduction and objective:
Peptic ulcer is a gastrointestinal disease that is characterized by mucosal damage. Nigella sativa (NS) is a medicinal plant increasingly used in practice for treating gastrointestinal diseases. The aim of the study was to evaluate the antiulcer potential of Nigella sativa oil in Albino rats.

Material and methods:
The study utilized two methods of gastric ulcer induction in Albino rats. The Aspirin-induced gastric ulcer method consisted of 3 groups, with 6 rats in each group, which received pre-treatment once a day orally for 5 days. Group-1 (Control) animals received distilled water. Group-2 (Standard) animals received the standard drug, Ranitidine. Group-3 (Expermintal) animals received the test drug – Nigella sativa oil. The forced swim induced acute gastric ulceration method consisted of 3 groups i.e., control, standard, and test group with 6 rats in each group. After 5 days of respective pre-treatment, the animals were subject to a forced swim test. On day 7, 4 hours after Aspirin ingestion, the animals were sacrificed by stunning. The stomachs of the sacrificed animals was dissected for ulcer scoring.

In the Aspirin-induced ulcer method, the percentage protection from ulcer formation was 47.5% in the Nigella sativa group and 52.9% in the standard group. The effect of Nigella sativa oil in comparison to control was statistically significant (p value=0.03). In the forced swim induced acute gastric ulceration method, the percentage protection offered by Nigella sativa oil was 73%, whereas it was 69.6% in the Ranitidine group, and the effect of Nigella sativa oil in comparison to the effect of control was statistically significant (p value=0.03).

The study demonstrated that NS oil has significant anti-ulcer potential in rats, which is comparable to the standard drug.

Shaima Zafer Khan, Ghulam Subhani, Ayesha Vaseem, Neeraj Sadiq, Mohammed Mohsin. Experimental evaluation of antiulcer potential of Nigella sativa oil in gastric ulcers in Albino rats. J Pre-Clin Clin Res. 2023; 17(1): 10–15. doi: 10.26444/jpccr/162337
Lanas A, Chan FKL. Peptic ulcer disease. Lancet. 2017;390:613–624. doi:10.1016/S0140-6736(16)32404-7.
Ramakrishnan K, Salinas RC. Peptic ulcer disease. Am Fam Physician 2007;76:1005–12.
Valle DL. Peptic ulcer diseases and related disorders. Harrison’s principles of internal medicine. 2005;16:1746–62.
Dajani EZ, Shahwan TG, Dajani NE. Overview of the preclinical pharmacological properties of Nigella sativa (black seeds): a complementary drug with historical and clinical significance. J Physiol Pharmacol. 2016;67:801–17.
Tavakkoli A, Mahdian V, Razavi BM, Hosseinzadeh H. Review on Clinical Trials of Black Seed (Nigella sativa) and its Active Constituent, Thymoquinone. J Pharmacopuncture. 2017;20:179–93.
Aggarwal BB, Kunnumakkara AB, Harikumar KB, Tharakan ST, Sung B, Anand P. Potential of spice-derived phytochemicals for cancer prevention. Plantamedica. 2008 Oct;74(13):1560–9.
Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, et al. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed. 2013;3:337–52.
Ahmad MF, Ahmad FA, Ashraf SA, Saad HH, Wahab S, Khan MI, Ali M, Mohan S, Hakeem KR, Athar MT. An updated knowledge of Black seed (Nigella sativa Linn.): Review of phytochemical constituents and pharmacological properties. Journal of herbal medicine. 2021 Feb 1;25:100404.
Arslan SO, Gelir E, Armutcu F, Coskun O, Gurel A, Sayan H, et al. The protective effect of thymoquinone on ethanol-induced acute gastric damage in the rats. Nutrit Res. 2005;25:673–80.
Sayed Masoud Hosseini, Elahe Taghiabadi, Khalil Abnous, Alireza Timcheh Hariri, Hamed Pourbakhsh, Hossein Hosseinzadeh. Protective effect of thymoquinone, the active constituent of Nigella sativa fixed oil, against ethanol toxicity in rats. Iran J Basic Med Sci. 2017 Aug;20(8):927–939.
Magdy MA, Hanan el-A, Nabila el-M. Thymoquinone: Novel gastroprotective mechanisms. Eur J Pharmacol. 2012;697(1–3):126–131.doi:10.1016/j.ejphar.2012.09.042.
Shahid F, Farooqui Z, Khan AA, Khan F. Oral Nigella sativa oil and thymoquinone administration ameliorates the effect of long-term cisplatin treatment on the enzymes of carbohydrate metabolism, brush border membrane, and antioxidant defense in rat intestine. Naunyn-Schmiedeberg’s archives of pharmacology, 2018. 391, 145–157.
Rajkapoor, et al. “Anti-Ulcer Effect of Nigella Sativa Linn. against Gastric Ulcers in Rats.” Current Science, vol. 82, no. 2, Temporary Publisher, 2002. pp. 177–79.
Wang Z, Hasegawa J, Wang X, Matsuda A, Tokuda T, Miura N, Watanabe T. Protective effects of ginger against aspirin-induced gastric ulcers in rats. Yonago acta medica. 2011 Mar;54(1):11.
Zeeyauddin K, Narsu ML, Abid M, Ibrahim M. Evaluation of antiulcer activity of Boswellia serrata bark extracts using aspirin induced ulcer model in albino rats. Journal of Medical & Allied Sciences. 2011 Jan 31;1(1).
Kulkarni K. Hand Book of Experimental Pharmacology. 3rd ed. New Delhi, India: Vallabh Prakashan; 2002.
Chaturvedi A, Kumar MM, Bhawani G, Chaturvedi H, Kumar M, Goel RK. Effect of ethanolic extract of Eugenia jambolana seeds on gastric ulceration and secretion in rats. Indian J Physiol Pharmacol. 2007;51(2):131–140.
Alarcon DL, Martin MJ, Lacasa C, Motivan V.Antiulcerogenic activity of flavonoids and gastric protection. Journal of Ethnopharmacology. 1994;42:161–170.
Al Mofleh IA, Alhaider AA, Mossa JS, Al-Sohaibani MO, Al-Yahya MA, Rafatullah S, Shaik SA. Gastroprotective effect of an aqueous suspension of black cumin Nigella sativa on necrotizing agents-induced gastric injury in experimental animals. Saudi J Gastroenterol. 2008 Jul;14(3):128–34. doi:10.4103/1319-3767.41731. PMID:19568521; PMCID: PMC2702910.
Al-Shaha OMS, Mohammed SA. Gastro protective effect of oil extract of Nigella sativa Seeds against Aspirin-Induced Gastric Ulcer in Albino Rats. J Entomol Zool Stud. 2017;5(4):725–732.
Sultana N, Khan MI, Ahmed N, Akhter MS, Momtaz A. Comparative Gastro-Protective Effects of Nigella sativa (Kalojira) and Omeprazole against Aspirin Induced Gastric Ulcer in Albino Rats. Delta Med Col J [Internet]. 2016 Aug. 19.
Kanter M, Demir H, Karakaya C, Ozbek H. Gastroprotective activity of Nigella sativa L oil and its constituent, thymoquinone against acute alcohol-induced gastric mucosal injury in rats. World journal of gastroenterology. 2005:11(42):6662–6666.
Guo S, Gao Q, Jiao Q, Hao W, Gao X, Cao JM. Gastric mucosal damage in water immersion stress: mechanism and prevention with GHRP-6.World J Gastroenterol. 2012 Jun 28;18(24):3145–55. doi:10.3748/wjg. v18.i24.3145. PMID: 22791951; PMCID: PMC3386329.
Robles TF, Carroll JE. Restorative biological processes and health. Soc Personal Psychol Compass. 2011;5:518–537.
Lin HP, Lin HY, Lin WL, Huang AC. Effects of stress, depression, and their interaction on heart rate, skin conductance, finger temperature, and respiratory rate: sympathetic-parasympathetic hypothesis of stress and depression. J Clin Psychol. 2011;67:1080–1091.
Koo MW, Ogle CW, Cho CH. Effects of verapamil, carbenoxolone and N-acetylcysteine on gastric wall mucus and ulceration in stressed rats. Pharmacology. 1986;32(6):326–34. doi: 10.1159/000138188. PMID: 3725888.
Mahathi K, Ramya MG, Samifar SK, Sindhuri TK, Madhuri K. Evaluation of anti-ulcer activity of methanolic extract of Leaves of Catharanthus roseus in experimental rats. Der Pharmacia Lettre. 2013;5(6):43–7.
Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res. 2000;14:323–8.
Journals System - logo
Scroll to top