Development and validation of a capillary electrophoresis method for determination of oxcarbazepine and zonisamide in pharmaceuticals
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Department of Medicinal Chemistry, Medical University, Lublin, Poland
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Beata Paw   

Department of Medicinal Chemistry, Medical University, Jaczewskiego 4, 20-090 Lublin, Poland.
J Pre Clin Clin Res. 2008;2(2):157-159
A simple, rapid capillary electrophoretic (CE) method has been evaluated and validated for determination of two newer antiepileptic drugs: oxcarbazepine and zonisamide, as pure substances and in tablet form. The method was developed by employing fused silica capillary with an effective length of 80 cm and internal diameter of 75 μm. 0.067 M phosphate buffer with pH 7.10 was used for oxcarbazepine analysis and 0.1 M acetate buffer with pH 4.58 was used for zonisamide determination. The recommended applied voltage, capillary temperature and injection time were 30 kV, 30°C and 10 s, respectively. Detection was followed by direct absorptiometric measurements at 210 nm (oxcarbazepine) or 240 nm (zonisamide). Developed methods were linear in the range of 5–100 μg/mL, and had good correlation coefficients (r=0.9999 for oxcarbazepine and r=0.9995 for zonisamide). Intra- and inter-day precision, calculated as relative standard deviation (RSD), were better than 2.0 %. The proposed CE methods were successfully applied to the assay of oxcarbazepine and zonisamide in pharmaceutical formulations.
Johannessen BD, Berry D, Bialer M, Kramer G, Tomson T, Patsalos P: Therapeutic drug monitoring of the newer antiepileptic drugs. Ther Drug Monit 2003, 25, 347-363.
Hachad H, Ragueneau-Majlessi I, Levy R: New antiepileptic drugs: review of drug interactions. Ther Drug Monit 2002, 24, 91-103.
Qi ML, Wang P, Wang LJ, Fu RN: LC method for the determination of oxcarbazepine in pharmaceutical preparations. J Pharm Biomed Anal 2003, 31, 57-62.
Ramaa CS, Chothe PP, Naik AA, Kadam VJ: Spectrophotometric method for the estimation of oxcarbazepine in tablets. Indian J Pharm Sci 2006, 68, 265-266.
Calvo ME, Renedo OD, Martinez MJ: Determination of oxcarbazepine by Square Wave Adsorptive Stripping Voltammetry in pharmaceutical preparations. J Pharm Biomed Anal 2007, 43, 1156-1160.
Paw B: Application of videodensitometric and classical densitometric thin-layer chromatography for quantifi cation of oxcarbazepine in pure and pharmaceutical preparations. JPCCR 2007, 1, 158-160.
Reddy TS, Devi PS: Validation of high-performance thin-layer chromatographic method with densitometric detection for quantitative analysis of two anticonvulsants in tablets. J Planar Chromatogr 2007, 20, 452-456.
Rao DVG, Chakravarthy IE, Kumar SR: Stability Indicating HPLC Method for the Determination of Zonisamide as Bulk Drug and in Pharmaceutical Dosage Form. Chromatographia 2006, 64, 261-266.
Pathare DB, Jadhav AS, Shingare MS: A Validated Stability-Indicating LC Method for Zonisamide. Chromatographia 2007, 66, 945-947.
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