Cell death and cytokine production induced by cadmium in leukocytes of cord blood and peripheral blood of adults
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Department of Virology and Immunology, Maria Curie-Skłodowska University, Lublin, Poland
Department of Cell Biology, Maria Curie-Skłodowska University, Lublin, Poland
Department of Gynaecology, Lublin District Hospital, Lublin, Poland
Corresponding author
Martyna Kandefer-Szerszeń   

Department of Virology and Immunology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.
J Pre Clin Clin Res. 2007;1(1):30-34
Cadmium has been shown to alter humoral and cell-mediated immune response in vivo and in vitro. The immune system of newborns is particularly vulnerable to the toxic effect of cadmium, but there is only limited knowledge concerning the influence of cadmium on cytokine production by the immune cells of newborns. Therefore, the aim of this paper was to compare the influence of cadmium on cell viability and cytokine production by cord blood cells and adult blood cells. The results of the study demonstrate that cord blood leukocytes were more sensitive than adult leukocytes to cadmium cytotoxicity, measured as the percent of cells undergoing apoptosis. Cord blood cells exhibited defects n PHA-and LPS-induced IL-2, IFN-γ and TNF-α production, compared with adult blood cells. Cadmium at 10 µM concentration enhanced IFN-γ production in cord blood cells and TNF-α production in the blood cells of adults; however, it decreased the production of IFN-α and IL-10 in both types of cells. The data show that cadmium-induced apoptosis of cord blood leukocytes, as well as enhanced production of pro-inflammatory IFN- γ, may result in deeper alterations in the immune cell functions of newborns after exposure to cadmium, than in adults.
Waalkes MP: Cadmium carcinogenesis in review. J Inorganic Biochem 2000, 79, 241-244.
Kantola M, Purkunen R, Kroger P, Tooming A, Juravskaja J, Pasanen M, Saarikoski, S, Vartiainen T: Accumulation of cadmium, zinc, and copper in maternal blood and developmental placental tissue: differences between Finland, Estonia and St. Petersburg. Environ Res 2000, 83, 54-66.
Truska P, Rosival L, Balazova G, Hinst J, Roppel A, Palusova O, Grunt J: Blood and placental concentration of cadmium, lead, and mercury in mothers and their newborns. J Hyg Epidemiol Microbiol Immunol 1989, 33, 141-147.
Moreno-Rojas R, Sachez-Segarra PJ, Amoro-Lopez MA, Zurera-Cosano. G: Cadmium content in infant formulas. Toxicological evaluation. Nahrung 2001, 45, 357-359.
Patriarca M, Lyon TD, Delves HY, Howatson AG, Fell GS: Determination of low concentrations of potentially toxic elements in human liver from newborns and infants. Analyst 1999, 124, 1337-1343.
Nishijo M, Nakagawa, H, Honda R, Tanebe K, Saito S, Teranishi H, Tawara K: Effects of maternal exposure to cadmium on pregnancy outcome and breast milk. Occup Environ Med 2002, 59, 394-397.
Miler I, Prochazkova E, Borte M, Vondracek, J, Braun W: Phagocytosis of cadmium microcrystals by glass-adherent peripheral blood leukocytes of newborns with various perinatal risk. Allerg Immunol , Leipzig 1987, 33, 231-237.
Miler I, Borte M, Vondracek J: Phagocytosis of cadmium microcristals by human milk macrophages in vitro. Allerg Immunol, Leipzig 1990 36, 157-162.
Marth E, Barth S, Jelovcan S: Influence of cadmium on the immune system. Description of stimulating reactions. Cent Eur J Publ Health 2000, 8, 40-44.
Marth E, Jelovcan S, Kleinhappl B, Gutschi A, Barth S: The effect of heavy metals on the immune system at low concentrations. Intl J Occup Med Environ Health 2001, 14, 375-386. 43.
Yucesoy B, Turhan A, Ure M, Imir T, Karakaya A: Simultaneous effects of lead and cadmium on NK cell activity and some phenotypic parameters. Immunopharmacol Immunotoxicol 1997, 19, 339-348.
Beyersmann D, Hachtenberg S: Cadmium, gene regulation, and cellular signaling in mammalian cells. Toxicol Appl Pharmacol 1997, 144, 247- 261.
Koropatnicki J, Zalups RK: Effect of non-toxic mercury, zinc or cadmium pretreatment on the capacity of human monocytes to undergo lipopolysaccharide-induced activation. Brit J Pharmacol 1997, 120, 797-806.
Hyun JS, Satsu H, Shimizu M: Cadmium induces interleukin-8 production via NF-kappaB activation in the human intestinal epithelial cell, Caco-2. Cytokine 2007, 37, 26-34.
Boscolo P, Di Giampoalo L, Qiao N, Reale M, Castellani ML, Lucci I, Travaglini P, Kouri M, Verna N, Volpe AR, Carmignani M, Paganelli R, Di Gioacchino M: Inhibitory effect of cadmium on peripheral blood mononuclear cell proliferation and cytokine release are reversed by zinc and selenium salts. Ann Clin Lab Sci 2005, 35, 115-120.
Hemdan NY, Emmrich F, Sack U, Wichman G, Lechmann J, Adham K, Lehmann I: The in vitro immune modulation by cadmium depends on the way of cell activation. Toxicol 2006, 222, 37-45.
Renvoise C, Biola A, Pallardt M, Breard J: Apoptosis: identification of dying cells. Cell Biol Toxicol 1998, 14, 111-120.
Lawrence S, Tang Y, Frank MB, Dozmorov I, Jiang K, Chen Y, Cadwell C, Turner S, Centola M, Jarvis JN: A dynamic model of gene expression in monocytes reveals differences in immediate/early response genes between adult and neonatal cells. J Inflamm 2007, 4, 1-19.
El-Mohandes AE, Brudno DS, Ahronovich MD: Impaired interferonalpha enhancement of neonatal monocyte phagocytosis. Biol Neonate 1990, 58, 260-263.
Trivedi HN, Hayglass KT, Gangur V, Allardice JG, Embree JE, Plummer FA: Analysis of neonatal T cell and antigen presenting cell functions. Human Immunol 1997, 57, 69-79.
D’Arena G, Musto P, Cascavilla N, Minervini MM, Di Giorgio G, Maglione A, Carotenuto M: Inability of activated cord blood T lymphocytes to perform Th1-like and Th2-like responses: implications for transplantation. J Hematother Stem Cell Res 1999, 8, 381-385.
Han P, Hodge G: Intracellular cytokine production and cytokine receptor interaction of cord mononuclear cells: relevance to cord blood transplantation. Brit J Haematol 1999, 107, 450-457.
Sautois B, Fillet G, Beguin Y: Comparative cytokine production by in vitro stimulated mononucleated cells from cord blood and adult blood xptl Hematol 1997, 25, 103-108.
Seghaye MC, Heyl W, Grabitz RG, Schumacher K, von Bernuth G, Rath W, Duchateau: The production of pro- and anti-inflammatory cytokines in neonates assessed by stimulated whole cord blood culture and by plasma levels at birth. Biol Neonate 1998, 73, 220-227.
Serushago B, Issekutz AC, Lee SHS, Rajaraman K, Bortolussi R: Deficient tumor necrosis factor secretion by cord blood mononuclear cells upon in vitro stimulation with Listeria monocytogenes. J Interferon Cytokine Res 1996, 16, 381-387.
Chalmers JM, Janossy G, Contreras M, Navarette C: Intracellular cytokine profile of cord and adult blood lymphocytes. Blood 1998, 92, 11-18.
Matsuda K, Tsutsumi H, Sone S, Yoto Y, Oya K, Okamoto Y, Ogra PL: Characteristics of IL-6 and TNF-alpha production by respiratory syncytial virus-infected macrophages in the neonate. J Med Virol 1996, 48, 199-203.
Miles EA, Bakewell L, Calder PC: Production of lymphocyte-derived cytokines by whole umbilical cord blood cultures stimulated with mitogens and allergens. Cytokine 2003, 21, 74-83.
Kruse A, Neustock P, Reuter M, Kirchner H: T-cell surface molecule expression and interferon-γ production in human cord blood. J Interferon Res 1993, 13, 221-225.
Lau AS, Sigaroudina M, Yeung M, Kohl S: Interleukin-12 induces interferon-γ expression and natural killer cytotoxicity in cord blood mononuclear cells. Pediatr Res 1996, 39, 150-155. Neustock P, Kruse A, Bock S, Pierre BS, Kirchner H: Deficient interferon- α response of newborns in comparison to adults. Lymphokine Cytokine Res 1993, 12, 109-114.
Clavell LA, Bratt MA: Relationship between the ribonucleic acidsynthesizing capacity of ultraviolet-irradiated Newcastle disease virus and its ability to induce interferon. J Virol 1971, 8, 500-508.
Chheda S, Palkowetz KH, Garofalo R, Rassin DK, Goldman AS: Decreased interleukin-10 production by neonatal monocytes and T cells: relationship to decreased production and expression of tumor necrosis factor-alpha and its receptors. Pediat Res 1996, 40, 475-483.
Kotiranta-Ainamo A, Rautonen R, Rautonen N: Interleukin-10 production by cord blood mononuclear cells. Pediatr Res 1997, 41, 110-113.
Marodi L: Deficient interferon-gamma receptor-mediated signaling in neonatal macrophages. Acta Paediatr 2002 (Suppl 91), 117-119.
Langrish CL, Buddle JC, Thrasher AJ, Goldblatt D: Neonatal dendritic cells are intrinsically biased against Th-1 immune responses. Clin Exptl Immunol 2002, 128, 118-123.
Feldman M, Brenan FM, Maini R: Cytokines in autoimmune disorders. Intl Rev Immunol 1998, 17, 217-28.
Kamiyama T, Miyakava H, Li JP, Akiba T, Liu JH, Marumo F, Sato C: Effects of one-year cadmium exposure on livers and kidneys and their relation to glutathione levels. Res Commun Mol Pathol Pharmacol 1995, 88, 177-186.
Kataranovski M, Kataranovski D, Savic D, Jovcic G, Bohdanovic Z, Jovanovic T: Granulocyte and plasma cytokine activity in acute cadmium intoxication in rats. Physiol Res 1998, 47, 453-461.
Souza V, Escobar M, Gomez-Quiroz L, Bucio L, Hernandez E, Cossio EC, Gutierrez-Ruiz MC: Acute cadmium exposure enhances AP-1 DNA binding and induces cytokine expression and heat shock protein 70 in HepG2 cells. Toxicol 2004, 197, 213-228.
De La Fuente H, Portales-Perez D, Baranda L, Diaz-Barriga F, Saavedra- Alanis V, Layseca E, Gonzales-Amaro R: Effect of arsenic, cadmium and lead on the induction of apoptosis of normal human mononuclear cells. Clin Exp Immunol 2002, 129, 69-79.
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