REVIEW PAPER
Viability of human Mesenchymal stem cells from the periradicular region at different concentrations of sodium hypochlorite – systematic review
1
Faculdade de Odontologia, Universidade de Pernambuco, Campus Santo Amaro, Pernambuco, Brasil. Programa de Pós-
Graduação em Odontologia, Universidade de Pernambuco, Pernambuco Recife, Brazil
Corresponding author
Michelle Almeida Silva
Faculdade de Odontologia, Universidade de Pernambuco, Campus Santo Amaro, Pernambuco, Brasil. Programa de Pós-Graduação em Odontologia, Universidade de Pernambuco, Pernambuco Recife, Campus Santo Amaro, Pernambuco, Brasil. Programa d, 58025-300, Recife, Brazil
Faculdade de Odontologia, Universidade de Pernambuco, Campus Santo Amaro, Pernambuco, Brasil. Programa de Pós-Graduação em Odontologia, Universidade de Pernambuco, Pernambuco Recife, Campus Santo Amaro, Pernambuco, Brasil. Programa d, 58025-300, Recife, Brazil
KEYWORDS
TOPICS
ABSTRACT
Pulp revascularization is the procedure that stimulates apical development and root maturation which is essential to secure
both survival and viability of stem cells for the success of the regenerative endodontic treatment. The aim of this review is to
investigate whether different concentrations of sodium hypochlorite (NaOCl) allow greater viability of human mesenchymal
stem cells (MSCs) from the periradicular region, without compromising root canals disinfection. The review is registered in
the PROSPERO database under protocol ID CRD42020203863. A search was conducted with no language restrictions in the
Medline/PubMed, Scopus, Web of Science databases, as well as a manual research, between March – June 2022, followed
by study selection and data extraction according to the PRISMA protocol. The search initially identified 999 articles, of
which 883 articles remained after the exclusion of duplicates. Of these, 11 were selected for full reading; 5 of which were
considered eligible to compose the qualitative synthesis. Articles that used human mesenchymal stem cells from the apical
papilla and periodontal ligament were included. The studies agreed that there is more than 50% loss of cell viability when
the culture is in contact with high concentrations (5–6%) of NaOCl after 10 minutes of contact. Low concentrations of NaOCl
(0.5–2.5%) provided 80–40% cell viability when in contact for 24 hours. The results of in vitro studies assessed in this review
indicate that low concentrations of NaOCl allow higher viability of stem cells from the periradicular region, therefore, they
are more recommended in cases of pulp revascularization.
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