RESEARCH PAPER
The presence of HPV DNA in breast cancer
| 1 | Departament of Virology, Medical University of Lublin, Poland |
| 2 | Departament of Oncology, Medical University of Lublin, Poland |
| 3 | Departament of Pneumonology, Oncology and Allergology, Medical University of Lublin, Poland |
CORRESPONDING AUTHOR
Paweł Macieląg
Departament of Virology, Medical University of Lublin, Chodźki 1, 20‑093 Lublin, Poland
Departament of Virology, Medical University of Lublin, Chodźki 1, 20‑093 Lublin, Poland
J Pre Clin Clin Res. 2013;7(2):86–89
KEYWORDS
ABSTRACT
Introduction and objective:
For many years the incidence of breast cancer remained at a high level. There are many risk factors which increase the probability of occurrence of the development of neoplastic transformation. Human papillomavirus has oncogenic properties and many studies have demonstrated its role in malignant transformation of cervical epithelial. These properties seems to be of great importance also in the formation of cancers in different organs.
Objective:
This study aims to assess the prevalence of HPV genetic material in women with breast cancer, and compare the prevalence of HPV DNA with a control group of women with non-malignant changes in the breast.
Material and Methods:
The biological material to be examined, obtained from 60 patients with breast cancer treated in Centrum Onkologii Ziemi Lubelskiej in Lublin, was fixed in paraffin blocks. The control group consisted of 30 patients with suspected tumour growth; however, the histopathological examination ruled out breast cancer cells. HPV DNA isolation was performed using a DNA Mini-Kit (Qiagen). Amplification and genotyping was carried out using a set of INNO-LIPA HPV Genotyping (Innogenetics) according to the manufacturer’s instructions.
Results:
The genetic material of the virus was detected in 8 women with breast cancer. DNA of HPV was not found in samples from the control group. HPV 16 occurred in 50%.
Conclusions:
HPV genetic material is present in breast cancer cells. HPV is absent in women with benign breast changes. A positive correlation was found between the presence of the HPV genome and the age of the women: the virus is more common in women aged > 60 years, also noted was the presence of HPV associated with the type of histopathological diagnosis. HPV is significantly more frequent in invasive lobular cancer.
For many years the incidence of breast cancer remained at a high level. There are many risk factors which increase the probability of occurrence of the development of neoplastic transformation. Human papillomavirus has oncogenic properties and many studies have demonstrated its role in malignant transformation of cervical epithelial. These properties seems to be of great importance also in the formation of cancers in different organs.
Objective:
This study aims to assess the prevalence of HPV genetic material in women with breast cancer, and compare the prevalence of HPV DNA with a control group of women with non-malignant changes in the breast.
Material and Methods:
The biological material to be examined, obtained from 60 patients with breast cancer treated in Centrum Onkologii Ziemi Lubelskiej in Lublin, was fixed in paraffin blocks. The control group consisted of 30 patients with suspected tumour growth; however, the histopathological examination ruled out breast cancer cells. HPV DNA isolation was performed using a DNA Mini-Kit (Qiagen). Amplification and genotyping was carried out using a set of INNO-LIPA HPV Genotyping (Innogenetics) according to the manufacturer’s instructions.
Results:
The genetic material of the virus was detected in 8 women with breast cancer. DNA of HPV was not found in samples from the control group. HPV 16 occurred in 50%.
Conclusions:
HPV genetic material is present in breast cancer cells. HPV is absent in women with benign breast changes. A positive correlation was found between the presence of the HPV genome and the age of the women: the virus is more common in women aged > 60 years, also noted was the presence of HPV associated with the type of histopathological diagnosis. HPV is significantly more frequent in invasive lobular cancer.
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