Metallothionein and manganese concentrations in breast cancer and mastopathic tissues
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Independent Laboratory for Cell Metabolism, Institute of Rural Health, Lublin, Poland
Oncological Centre, Lublin, Poland
Department of Bromatology, Medical University, Lublin, Poland
Corresponding author
Bolesław Floriańczyk   

Independent Laboratory for Cell Metabolism, Institute of Rural Health, Jaczewskiego 2, 20-950 Lublin, Poland.
J Pre Clin Clin Res. 2011;5(2):63-65
Metallothionein (MT) takes part in the homeostasis of the metals which are necessary for proper metabolism, and they also take part in the detoxication of toxic metals from the tissues. Additionally, they protect the tissues from the eff ects of reactive, oxygen species, radiation, mutagens, and from electrophilic pharmacologic agents used in cancer therapy. Manganese (Mn) – an essential microelement - seems to be very important during development. Mn takes a part as cofactor of the enzymes regulating metabolism. This microelement also participates in reduction-oxidation reactions. An example of such a reaction is the superoxide dismutase (MnSOD) activity, with manganese as the enzyme component. It is thought that manganese and manganese superoxide dismutase play an important role in the diff erentiating cells. The aim of this work was to evaluate whether the neoplastic process changes the values of metallothionein and manganese. The examined material consisted of samples of breast tumour taken during surgery. The level of the metallothionein was determined by the cadmium- hemoglobin affi nity assay, using the cadmium isotope (109Cd); the concentration of manganese was determined by atomic absorption spectrometry. It was found that metallothionein and manganese concentration in breast cancer was higher in comparison with mastopathic lesions of the breast. Neoplastic cells contained more metallothioneins (by 330%) and more manganese (by 25%). Negative correlations were found between the amount of metallothionein and the level of manganese in mastopathic lesions (r = -0.57, p < 0.05), and in breast cancer (r = -0.32, p > 0.05). The relatively high level of metallothionein and manganese in the neoplastic tissues indicates the important role may play in the metabolism of cancer cells. Negative correlation between the concentration of metallothionein and manganese ions found in mastopathic tissues and breast cancer indicates that their roles are opposite.
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