Using PET/CT imaging performance to qualify 18 F-Fluorodeoxy- glucose (FDG) uptake in common carp (Cyprinus carpio)
More details
Hide details
Department of Animal Husbandry, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Hungary
Department of Nuclear Medicine of Medical and Health Centre, PET-CT Center, University of Debrecen, Hungary
J Pre Clin Clin Res. 2016;10(1):60–62
Positron Emission Tomography (PET) is a non-invasive diagnostic tool that provides tomographic images and measures quantitative parameters of cell viability and metabolic activity of tissues. The most important used tracer in PET is an 18-F-Fluorodeoxy-glucose (FDG), the glucose molecule of which is labeled with a radiotracer and allows measurement and mapping of tissue glucose uptake. There are many studies in PET/CT which rely on some mammalian species, and recently on fish.

The aim of this survey by using FDG-PET/CT are to optimize and determine FDG uptake in fish, in this case, common carp (Cyprinus carpio) using three treatments: Basic fish meal, Vita Pulvis and Probiotics s with two replications were used. Sphere Volume of interest (VOI) were drawn hand for the all organs, and standard uptake value (SUV) means were calculated.

The SUV mean for glucose uptake in the liver and gastrointestinal tract of fish were more similar to those of humans than rats or mice. SUV mean in fish fed by Probiotics ss in the major organ were less than those fed by Vita Pulvis and basic fish meal. The results present the opportunity to focus on studies of metabolism and screening for the effects of nutrients on body development.

Sabouran Zaheri Abdehvand   
Department of Animal Husbandry, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Boszormenyi 138, 1/134 4032 Debrecen, Hungary
1. Lucké B, Schlumberger H. Transplantable epitheliomas of the lip and mouth of catfish I. Pathology. Transplantation to anterior chamber of eye and into cornea. J Exp Med. 1941; 74: 397–408.
2. Bailey GS, Williams DE, Hendricks JD. Fish models for environmental carcinogenesis: the rainbow trout. Environ Health Perspect. 1996; 104: 5–21.
3. Okihiro MS, Hinton DE. Progression of hepatic neoplasia in Medaka (Oryzias latipes) exposed to diethylnitrosamine. Arcinogenesis 1999; 20: 933–940.
4. Stern HM, Zon LI. Cancer genetics and drug discovery in the Zebrafish. Nat Rev Cancer. 2003; 3: 533–539.
5. Berghmans S, Jette C, Langenau D, Hsu K, Stewart R, Look T, Kanki JP. Making waves in cancer research: new models in the Zebrafish. BioTechniques. 2005; 39: 227.
6. Kissling GE, Bernheim NJ, Hawkins WE, Wolfe MJ, Jokinen MP, Smith CS, Herbert RA, Boorman GA. The utility of the guppy (Poecilia reticulata) and Medaka (Oryzias latipes) in evaluation of chemicals for carcinogenicity. Toxicol Sciences 2006; 92: 143–156.
7. Goessling W, North TE, Zon LI. New waves of discovery: modeling cancer in zebrafish. J Clin Oncol. 2007; 25: 2473–2479.
8. Taylor AM, Zon LI. Zebrafish tumor assays: the state of transplantation. Zebrafish 2009; 6:339–346.
9. Lee BY, Howe AE, Conte MA, D’Cotta H, Pepey E, Baroiller JF. An EST resource for tilapia based on 17 normalized libraries and assembly of 116,899 sequence tags. BMC Genomics. 2010; 11: 278.
10. Fraser BA, Weadick CJ, Janowitz I, Rodd FH, Hughes KA. Sequencing and characterization of the guppy (Poecilia reticulata) transcriptome. BMC Genomics. 2011; 12: 202.
11. Oh ES, Park SH, Chang YT, Kim CH, Choi SY, Williams DR. A novel Zebrafish human tumor xenograft model validated for anti-cancer drug screening. Mol Biosyst. 2012; 8: 1930–1939.
12. Pickart, MA, Klee EW. Zebrafish approaches enhance the translational research tackle box. Transl Res. 2014; 163: 65–8.
13. Browning ZS, Wilkes AA, MacKenzie DS, Patterson RM, Lenox MW. Using PET/CT imaging to characterize 18 F-fluorodeoxyglucose utilization in fish. J Fish Dis. 2013; 36: 911–919.
14. Wilkes AA, Browning ZS, Lenox M, Jaques J, Mackenzie DS. High resolution functional imaging of fish endocrine glands and target tissues using positron emission tomography-computed tomography. Society for integrative and comparative biology, Annual meeting; 2014.
15. Djang M, Lieberman G. PET/CT: Basic Principles, Applications in Oncology; 2006 http://eradiology.bidmc.harvar... gastro/Djang.pdf.
16. Kosuda Sh, Fisher S, Kison PV, Wahl RL, Grossman HB. Uptake of 2-deoxy-2-[18F]fluoro-D-glucose in the normal testis: Retrospective PET study and animal experiment. Ann Nuclear Med. 1977; 11(3): 195–199.
17. Boss DS, Olmos RV, Sinaasappel M, Beijnen JH, Schellens JHM. Application of PET/CT in the development of novel anticancer drugs. The Oncologist. 2008; 13: 25–38.
18. Hutchinson O, Collingridge DR, Barthel H, Price PM, Aboagye EO. Pharmacodynamics of radiolabelled anticancer drugs for positron emission tomography. Curr Pharmaceutical Des. 2003; 9: 931–944.
19. Liu P, Huang G, Dong S, Wan L. Kinetic analysis of experimental rabbit tumour and inflammation model with 18F-FDG PET/CT. Nuklearmedizin. 2009; 48: 153–158.
20. Landau BR, Spring-Robinson CL, Muzic RF, Rachdaoui N, Rubin D, Berridge MS, Schumann WC, Chandramouli V, Kern TS, Ismail-Beigi F. 6-Fluoro-6-deoxy-D-glucose as a tracer of glucose transport. Am J Physiol-Endocrinol Metab. 2007; 293: E237–E245.
21. MacKenzie DS, Vanputte CM, Leiner KA. Nutrient regulation of endocrine function in fish. Aquaculture. 1998; 161; 3–25.
22. Ryan Gregory T, Wood CM. The effects of chronic plasma cortisol elevation on the feeding behavior, growth, competitive ability and swimming performance of juvenile rainbow trout. Physiol Biochem Zool. 1999; 72(3); 286–295. Pierce AL, Shimizu M, Beckman BR, Baker DM, Dickhoff WW. Time course of the GH/IGF axis response to fasting and increased ration in chinook salmon (Oncorhynchus tshawytscha). Gen Comp Endocrinol. 2005; 140: 192–202.
23. Trichet VV. Nutrition and immunity: an update. Aquacult Res. 2010; 41: 356e72.
24. Rawling MD, Merrifield DL, Snellgrove DL, Kühlwein H, Adams A, Davies SJ. Haemato-immunological and growth response of mirror carp (Cyprinus carpio) fed a tropical earthworm meal in experimental diets. Fish Shellfish Immunol. 2012; 32: 1002–1007.
25. Neiffer DL, Stamper MA. Fish sedation, anesthesia, analgesia, and euthanasia: considerations,methods, and types of drugs. ILAR J. 2009; 50: 343–360.