RESEARCH PAPER
Reactivation of BKV and AdV infections during post-transplant immunosuppressive therapy
 
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1
Department of Virology, Medical University of Lublin
2
Department of Ethics and Human Philosophy, Medical University of Lublin
CORRESPONDING AUTHOR
Adrian Jarzyński   

Department of Virology, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland
 
J Pre Clin Clin Res. 2013;7(2):93–97
KEYWORDS
ABSTRACT
Introduction and Objectives.:
Viral infections are an important problem in transplantology. Infections in recipients may develop as a result of the original infection or reactivation of a latent infection. Many viruses have the ability to enter into a latent state without symptoms. The most common groups of viruses causing infections in patients after transplantations are herpersviruses, hepatotropic viruses, retroviruses, adenoviruses and poliomaviruses. The aim of the presented study is to analyze the incidence of infections caused by BKV and AdV in a group of patients after kidney and bone marrow transplant.

Material and Methods:
The study group consisted of 13 patients after bone marrow transplantation and a group of 10 patients after kidney transplantation. DNA was isolated from urine and serum and analysed with PCR auto-nested reaction. The amplification products were separated in agarose gel.

Results:
A positive result for AdV was recorded in 7 patients after bone marrow transplant and 5 patients after renal transplant. BK virus was detected in the urine of 13 patients, and in the blood sample of 5 patients. Reactivation of a virus during immunosuppression is a frequent cause of graft rejection. In addition, in patients with impaired immune system, mixed infections with various types of viruses are becoming a serious problem. This study confirm that infections with BKV and AdV viruses are a major problem in the field of transplantation.

Conclusions:
BKV and AdV are common causes of infections affecting patients after renal and bone marrow transplantation. BKV infections are often accompanied by human adenovirus infection. Adenovirus infections are a more common cause of infection in bone marrow transplant patients than in patients after renal transplantation.

 
REFERENCES (25)
1.
Skrzypek A. Infectious complications after kidney transplantation. Przegląd urologiczny 2007; 8: 41.
 
2.
Echavarria M. Adenoviruses in immunocompromised host. ClinMicrobiol Rev. 2008; 21: 705–715.
 
3.
Hu JH, Zhao H, Huang YP, Zhang X. Oportunistic posttransplantation virus infections in renal transplantant recipients. Transplant Proceed. 2011; 43: 3715–3719.
 
4.
Collier L, Oxford J. Human Virology. 3th Ed. PZWL. Warsaw, 1996. p.102–146.
 
5.
Kańtoch M. Medical Virology. 2nd Ed. PZWL. Warsaw, 1998. p.68–187.
 
6.
Dzieciątkowski T, Rola A, Midak-Siewirska A. Adenoviral infection of humans. Post Mikrobiol. 2008; 47: 15–22.
 
7.
Kotloff KL, Losansky GA, Morris JG Jr, et al. Enteric adenovirus infectionand childhood diarrhea: an epidemiologic study in three clinical setting. Pediatrics. 1989; 84: 219–225.
 
8.
Butt AL, Chodosh J. Adenoviral keratoconjuncitivitis in a tretiary care eye clinic. Cronea. 2006; 25: 199–201.
 
9.
Shindo K, Kitayama T, Ura T, et al. Acute hemorrhagic cystitis caused by adenovirus type 11 after transplantation. Urologia Internationalis. 1986; 41: 152–155.
 
10.
Ryanans S, Dzieciątkowski T, Młynarczyk G. Human polyoma viruses infection in immunosuppressed patients. Post Mikrobiol. 2011; 50: 191–199.
 
11.
Boothpur R, Brennan DC. Human polyoma viruses and disease with emphasis on clinical BK and JC. J Clin Virol. 2010; 47: 306–312.
 
12.
Jeffers LK, Madden V, Webster-Cyriaque J. BK virus has tropism for human salilary gland cells in vitro: implications for transmission. Virology. 2009; 394: 183–193.
 
13.
Garnett CT, Pao CI, Gooding LR. Detection and quantitation of subgroup C adenovirus DNA in human tissue samples by real-time PCR. Methods Mol Med. 2007; 130: 193–204.
 
14.
Lion T, Baurngartiner R, Watzinger F, et al. Molecular monitoring of adenovirus in peripheral blood after allogeneic bone marrow transplantation permits early diagnosis of disseminated disease. Blood. 1003; 60: 133–140.
 
15.
Leen AM, Bollard CM, Myers GD, Rooney CM. Adenoviral infections in hematopoietic stem cell transplantation. Biol Blood Marrow Transplant. 2006; 12: 243–251.
 
16.
Flomenberg P, Babbit J, Drobyski WR, et al. Increasing incidence of adenovirus disease in bone marrow transplant recipients. I Infect Dis. 2004; 169: 775–781
 
17.
Watcharananan SP, Aver R, Ingsathit A, Malathum K. Adenovirus Disease after Kidney Transplantation: Course of infection and Outcome in Relation to Blood Viral and Immune Recovery. Am J Transplant. 2011; 11: 1308.
 
18.
Bil-Lula I, Ussowicz M, Rybka B, et al. PCR diagnostic and monitoring of adenoviral infections in hematopoetic stem sell transplantation recipients. Arch of Vir. 2010; 155: 2007.
 
19.
Pang XL, Doucette K, LeBlanc B. Monitoring of polyomavirus BK Viruria and Viremia in Renal Allograft Recipients by Use of a QQuantative Real-Time PCR Assay: One-Year Prospective Study. J Clin Microbiol. 2007; 45: 3568.
 
20.
Matłosz B, Durlik M. Interstitial nephritis kidney transplant Polyoma BK virus-induced. Przegląd Epidemiologiczny. 2006; 60: 133–140.
 
21.
Khaled AS. Polyomavirus (BK virus) Nephropathy in Kidney Transplant Patients: A Phatologic Precpective. Yonsei Med J. 2004; 45: 1066.
 
22.
Cimbaluk D, Pitelka L, Kluskens L, Gattuso P. Update on human polyoma virus BK nephropathy. Diag Cytopathol. 2009; 37: 773.
 
23.
Sung H, Choi BH, Jung Y, Kim M. Quantitation of BK Virus DNA for Diagnosis of BK Virus-Associated Nephropathy in Renal Transplantant Recipients. J Korean Med Sci. 2008; 23: 814–818.
 
24.
24. Giruad G, Priftakis P, Bogdanovic G, et al. BK-viruria and haemorrhagic cystitis are more frequent an allogeneic hematopoietic stem cell transplant patients receiving full conditioning and unrelated HLAmismatched grafts. Bone Marrow Transplant. 2008; 41: 737–742.
 
25.
Giraud G, Bogdanovic G, Priftakis P, Remberg M. The incidence of hemorrhagic cystitis and BK-viruria in allogeneic hematopoietic stem cell recipients according to intensity of conditioning regiment. Hematologica. 2006; 91: 401–404.
 
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