No eff ect of water extract of Scutellariae radix on the anticonvulsant action of valproate, tiagabine and topiramate in two animal models of epilepsy
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Department of Physiopathology, Institute of Agricultural Medicine, Lublin, Poland
Department of Pathophysiology, Medical University, Lublin, Poland
JPCCR 2009;3(2):95–98
Water extract of Scutellariae radix (SR) was previously reported to display a signifi cant anticonvulsant eff ect on maximal electroshock (MES)-induced seizures, and little anticonvulsant eff ect in pentylenetetrazole (PTZ)-induces clonic seizure model in mice. The aim of this study was to examine whether water extract of SR has any impact on anticonvulsant properties of valproate (VPA) and tiagabine (TGB) in PTZ-induced clonic seizure model in mice and VPA and topiramat (TPM) in the mouse MES test. Results indicated that water extract of SR did not signifi cantly aff ect the anticonvulsant action of VPA and TPM against MES-induced tonic seizures. The experimentally derived median eff ective doses (ED50 values) for VPA administered alone and in combination with water extract of SR were 219 and 203 mg/kg and for TPM administered alone, and in combinations with water extract of SR were 41.6 and 40.8 mg/kg, respectively. Likewise, no eff ect of water extract of SR was observed on VPA and TGB in PTZ-induced clonic seizure models. The ED50 values for TGB administered alone and in combination with water extract of SR were 0.84 and 0.71 mg/kg, while those for VPA were 158 and 129 mg/kg, respectively. In conclusion, water extract of SR had no eff ect on the anticonvulsant activity of TPM and VPA in the mouse MES seizure model and on the antiseizure activity of VPA and TGB in PTZ-induced clonic seizures in mice.
Marta Andres-Mach   
Department of Physiopathology, Institute of Agricultural Medicine, Jaczewskiego 2, 20-090 Lublin, Poland.
1. Lin CC, Shieh DE: The anti-infl ammatory activity of Scutellaria rivularis extract and its active components, baicalin, baicalein, and wogonin. Am J Chin Med 1996, 24, 31-36.
2. Nagai T, Suzuki Y, Tomimori T, Yamada H: Antiviral activity of plant fl avonoid, 5,7,4’-trihydroxy-8-methoxyfl avone, from the roots of Scutellaria baicalensis against infl uenza A (H3N2) and B viruses. Biol Pharm Bull 1995, 18, 295-299.
3. Kimura Y, Yokoi K, Matsushita N, Okuda H: Eff ects of fl avonoids isolated from scutellariae radix on the production of tissue-type plasminogen activator and plasminogen activator inhibitor-1 induced by thrombin and thrombin receptor agonist peptide in cultured human umbilical vein endothelial cells. J Pharm Pharmacol 1997, 49, 816-822.
4. Gao Z, Huang K, Yang X, Xu H: Free radical scavenging and antioxidant activities of fl avonoids extracted from the radix of Scutellaria baicalensis Georgi. Biochim Biophys Acta 1999, Nov 16,1472(3), 643-650.
5. K, Komatsu Y: Inhibitory eff ect of Coptidis Rhizoma and Scutellariae Radix on azoxymethane-induced aberrant crypt foci formation in rat colon. Biol Pharm Bull 1998, 21(8), 814-817.
6. Chan FL, Choi HL, Chen ZY, Chan PS, Huang Y: Induction of apoptosis in prostate cancer cell lines by a fl avonoid, baicalin. Cancer Lett 2000, 160(2),219-228.
7. Wang HH, Liao JF, Chen CF: Anticonvulsant eff ect of water extract of Scutellariae radix in mice. J Ethnopharmacol 2000, Nov, 73(1-2),185- 190.
8. Wang F, Xu Z, Ren L, Tsang SY, Xue H: GABA A receptor subtype selectivity underlying selective anxiolytic eff ect of baicalin. Neuropharmacology 2008, 55(7),1231-1237.
9. Loscher W, Nolting B: The role of technical, biological and pharmacological factors in the laboratory evaluation of anticonvulsant drugs. IV. Protective indices. Epilepsy Res 1991, 9(1), 1-10.
10. Loscher W, Schmidt D: Which animal models should be used in the search for new antiepileptic drugs? A proposal based on experimental and clinical considerations. Epilepsy Res 1988, 2(3),145-181.
11. Łuszczki JJ: Isobolographic analysis of interaction between oxcarbazepine and valproate in pentylenetetrazole-induced seizures in mice. J Pre-Clin Clin Res 2008, 2, 40-45.
12. Łuszczki JJ, Krzyżanowski M, Sielski M, Wojda E, Świąder M: Interaction of tiagabine with clonazepam in the mouse pentylenetetrazole-induced seizure model: a type I isobolographic analysis for parallel log-probit dose-response relationship lines. J Pre-Clin Clin Res 2008, 2, 141-146.
13. Łuszczki JJ, Zadrożniak A, Barcicka-Kłosowska B, Bednarski J, Misiuta-Krzesińska M, Filip D, Zwoliński J, Czernecki R: Infl uence of 7-nitroindazole and NG-nitro-L-arginine on the anticonvulsant activity of loreclezole in maximal electroshock-induced seizures in mice. J Pre-Clin Clin Res 2007, 1, 146-149.
14. Litchfi eld JT, Wilcoxon F: A simplifi ed method of evaluating dose-eff ect experiments. J Pharmacol Exp Ther 1949, 96(2), 99-113.
15. Liao JF, Jan YM, Huang SY, Wang HH, Yu LL, Chen CF: Evaluation with receptor binding assay on the water extracts of ten CNS-active Chinese herbal drugs. Proc Natl Sci Counc Repub China B 1995, 19(3), 151-158.
16. Łuszczki JJ, Andres-Mach M, Cisowski W, Mazol I, Głowniak K, Czuczwar SJ: Osthole suppresses seizures in the mouse maximal electroshock seizure model. Eur J Pharmacol 2009, 607(1-3), 107- 109.
17. Łuszczki JJ, Wojda E, Andres-Mach M, Cisowski W, Gleńsk M, Głowniak K, Czuczwar SJ: Anticonvulsant and acute neurotoxic eff ects of imperatorin, osthole and valproate in the maximal electroshock seizure and chimney tests in mice: a comparative study. Epilepsy Res 2009, 85(2-3), 293-299.
18. Łuszczki JJ, Głowniak K, Czuczwar SJ: Time-course and dose-response relationships of imperatorin in the mouse maximal electroshock seizure threshold model. Neurosci Res 2007, 59(1), 18-22.