REVIEW PAPER
Electric Cell Substrate Impedance Sensing (ECIS) as a unique technique in cancer metastasis research
 
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1
Physiology Department, Medical University, Lublin, Poland
2
Independent Public Health Care Centre, Radzyń Podlaski, Poland
3
1st Military Clinical Hospital, Lublin, Poland
4
Department of Molecular Biology and Translational Research, Institute of Rural Health, Lublin, Poland
CORRESPONDING AUTHOR
Monika Prendecka   

Physiology Department, Medical University, Lublin, Poland, Radziwiłłowska 11 Str., 20-080, Lublin, Poland
 
J Pre Clin Clin Res. 2018;12(4):142–144
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Metastasis is defined as the ability of cancer cells to migrate from the site of primary tumour growth to a distant location. The metastatic tumour is usually more lethal than the initial primary cancer which is the reason for the metastatic cancer being more lethal. This cancer metastasis study gives a very important insight into how invasive cells cross the endothelial and epithelial monolayers. By simultaneously monitoring both barrier function and cell viability, Electric Cell Substrate Impedance Sensing (ECIS®) can distinguish between the transmigration mechanisms that leave the monolayer intact from those that disrupt the cell layer.

Objective:
The aim of the study is to show the unique (ECIS) technique as aq useful tool in the study of cancer metastasis.

Discussion:
Intensive research on the method development is the reason for attempts to explain the relationship between electrical changes in cells or on their surface, and the processes influencing survival, which require a holistic approach. As a result of new technical possibilities, including ECIS, and assuming that changes in the electrical properties of cells precede changes on the biochemical level, it would be very interesting to examine the character and dynamics of these changes. The electric parameters measured by the ECIS system are impedance, resistance and capacitance. All of them can be used to examine cell transformation, migration and invasion, cell proliferation and tight junctions as a function of barrier resistance.

Conclusions:
As cell function modulates cell morphology, ECIS is a method capable of detecting and quantifying for recording morphology changes in the subnanometer to micrometer range. This study describes the possibility of using ECIS in metastasis research.

 
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