Characteristics of gut microbiome of obese middle-aged men in the Lublin region – preliminary report
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Division of Cardiology with Cardiac Intensive Care Unit, Cardinal Stefan Wyszyński Hospital, Lublin, Poland
Pope John Paul II State School of Higher Education, Biała Podlaska, Poland
Division of Gastroenterology, Cardinal Stefan Wyszyński Hospital, Lublin, Poland
Anna Gózd-Barszczewska   

Stefan Cardinal Wyszyński Provincial Specialist Hospital, al. Kraśnicka 100, 20-718 Lublin, Poland
J Pre Clin Clin Res. 2019;13(1):37–41
Introduction and Objective:
Overweight and obesity are among the main health problems in highly developed countries, including Poland. An effective fight against complications of these phenomena may be the basis for prevention and improvement of therapy of many diseases. This preliminary study is an attempt to determine the composition of the intestinal microflora in the local population of obese middle-aged men particularly exposed to the adverse consequences of overweight and obesity.

Material and methods:
Stool samples of 20 men with abdominal obesity were the material for examination. The composition of microbiome was determined using the next generation sequencing method targeting the V3-V4 hipervariable region of 16S rRNA. The Greengenes gene collection was used as a reference database.

This was the first attempt to determine the gut microbiome composition in a local population. The predominant components of microbiota were bacteria classified as Firmicutes (47.45%) and Bacteroidetes (46.05%). The most abundant Class were Clostridia (47.06%) and Bacteroidia (46.05%), and most abundant Order were Clostridiales (46.50%) and Bacteroidales (45.93%). The greatest diversity concerned the Genus category in which the most abundant groups of microorganisms were bacteria belonging to Bacteroides (26.57%) and Prevotella (13.26%)

Molecular sequencing methods allow determination of the composition of microbiome, including microorganisms that can't be identified using classical diagnostic methods. The obtained results may form the basis for further research, including larger groups of participants, aimed at determining the role of microbiota in the development of abdominal obesity and its complications. The results can also be used for comparative studies with those from other regions, and be a part of global efforts to determine human ‘core microbiome’.

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