RESEARCH PAPER
Application of high performance thin layer chromatography method for ophthalmological preparations containing anthocyanins fractions
 
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1
Department of Chemistry, Laboratory of Planar Chromatography, Medical Academy, Staszica 6, 20-081, Lublin, Poland
2
II Clinic of Ophthalmology, Medical Academy, Chmielna 3, 20-079, Lublin, Poland
3
Department of Vascular Surgery, Medical Academy, Staszica 3, 20-081, Lublin, Poland
 
J Pre Clin Clin Res. 2007;1(1):35–38
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ABSTRACT
The aim of the investigation was to elaborate a fast, easy, and cheap method of analysis of ophthalmological preparations containing anthocyanins fractions. A high performance thin layer chromatography (HPTLC) was applied; the chromatographic conditions were determined experimentally. Eight preparations with extracts of Vaccinium myrtillus L. and Aronia melanocarpa L. as reference material were analyzed. In the optimal chromatographic system, with methyl tert-butyl ether as the modifier of mobile phase, the separation of anthocyanins fractions was very good. The mobile phase enables separation of natural anthocyanins fractions from synthetic pigments (e.g., methylene blue).
 
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