RESEARCH PAPER
Antinociceptive screening of various 1,2,4-triazole-3-thione derivatives in the hot-plate test in mice
 
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1
Department of Pathophysiology, Medical University of Lublin, Poland
2
Isobolographic Analysis Laboratory, Institute of Rural Health, Poland
3
Department of Pharmacology, Medical University of Lublin, Poland
CORRESPONDING AUTHOR
Jarogniew J. Łuszczki   

Department of Pathophysiology, Medical University of Lublin, Department of Pathophysiology, 20-090, Lublin, Poland
 
J Pre Clin Clin Res. 2019;13(1):9–12
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Despite the large number of analgesic drugs available currently, pain therapy is still a challenging issue for researchers and clinicians. The search for new drugs that could relieve patients from pain is not only justified, but also highly recommended.

Objective:
This study aimed to perform antinociceptive screening of 4 various 1,2,4-triazole-3-thione derivatives (TPB-2, TPB-4, TPF-32 and TPF-38) in the hot-plate test in mice, which is an experimental model allowing the testing of compounds alleviating acute thermal pain.

Material and methods:
Experimental verification of the antinociceptive effects of the tested compounds (administered intraperitoneally in a constant dose of 300 mg/kg) was performed in the hot-plate test in mice, by calculating maximum possible antinociceptive effects (MPAE in %) at 4 various pretreatment times (15, 30, 60 and 120 min.).

Results:
TPB-2 exerted strong antinociceptive effects with MPAE ranging between 18.54 – 35.43% in the hot-plate test. Similarly, TPF-32 exerted firmly established antinociceptive effects with MPAE ranging from 13.50 – 37.05%. In the case of TPB-4 and TPF-38, both compounds produced slight changes in MPAE in the hot-plate test in mice. These agents can be classified as virtually ineffective in the hot-plate test.

 
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