Influence of prolonged manganese intoxication on memory processes in hypoxic mice
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Department of Physiopathology, Institute of Agricultural Medicine, Lublin, Poland
Department of Hygiene, Medical University, Lublin, Poland
Krzysztof Łukawski   

Department of Physiopathology, Institute of Agricultural Medicine, Jaczewskiego 2, 20-090 Lublin, Poland.
J Pre Clin Clin Res. 2010;4(1):43–46
Exposure to high levels of manganese in the workplace can result in the development of neurotoxic symptoms in humans. It is also known that exposure to heavy metals, including manganese, can lead to learning and memory deficits. Recently, it has been demonstrated that hypoxic mice in the model of bilateral clamping of the carotid arteries (BCCA) can be more vulnerable to the effects of lead or cadmium. The purpose of the present study was to examine the effect of prolonged manganese intoxication – up to 10 days – on memory processes in mice exposed to transient cerebral oligemic hypoxia induced by 30 min of BCCA. In the current study, long-term memory was evaluated using the step-through passive avoidance task. Spatial working memory was assessed by recording spontaneous alternation in the Y-maze test. In the passive avoidance task, manganese administered at a dose of 7.95 mg/kg i.p. did not impair retention in BCCA mice. Manganese at the same dose of 7.95 mg/kg i.p. did not alter alternation behaviour in the Y-maze either. These findings suggest that hypoxia induced by BCCA combined with prolonged manganese intoxication does not affect memory functions of mice.
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