RESEARCH PAPER
Healthy ageing: the beneficial effect of dietary supplementation with alpha-ketoglutarate on arterial elasticity in elderly mice
| 1 | Department of Animal and Veterinary Basic Sciences, Faculty of Life Sciences, Copenhagen University, Frederiksberg, Denmark |
| 2 | Department of Food Science, Faculty of Life Sciences, Copenhagen University, Frederiksberg, Denmark |
| 3 | Copenhagen University Library and The Parker Institute, Frederiksberg Hospital, Denmark |
| 4 | Institute of Agricultural Medicine, Department of Medical Biology, Lublin, Poland, Department of Cell and Organism Biology, Lund University, Sweden |
CORRESPONDING AUTHOR
Adrian P. Harrison
IBHV, LIFE, Copenhagen University, Grønnegaardsvej 7, 1870 Frederiksberg C, Denmark.
IBHV, LIFE, Copenhagen University, Grønnegaardsvej 7, 1870 Frederiksberg C, Denmark.
J Pre Clin Clin Res. 2009;3(1):24–30
KEYWORDS
ABSTRACT
The study employed mechanical stretching in vitro of sections of abdominal aorta of elderly mice to investigate any benefi ts of oral treatment with alpha-ketoglutarate (AKG) on arterial elasticity. Eighteen female mice (50-weeks-old) were assigned to a control (2% w/v) Na2-AKG or (2% w/v) a Ca-AKG group, and treated for 182 days before being humanely killed. Aorta sections were exposed to increases in force (0.09 N) giving approx. 6 kPa of pressure, after which elastic recoil (N ms-1 mg-1 wet wt.) was measured. Na2-AKG treatment for 182 days induced a 30% (P <0.01)
improvement, while Ca-AKG induced a 93% (P <0.001) improvement in the elastic recoil compared to controls (3.30 ± 0.08 × 10-5 N ms-1 mg-1 wet wt). AKG administered orally improved arterial elasticity in elderly mice, a change that occurred despite an increase in total collagen content. Alpha-ketoglutarate warrants further investigation as a candidate for therapies targeting arterial stiff ening with age.
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