The eff ects of increased inspired oxygen with and without dopamine on lung and diaphragm hydrogen peroxide and apoptosis following hemorrhagic shock
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School of Nursing, University of Kansas, Kansas City, USA
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas, Kansas City, USA
Amanda R. Thimmesch   

School of Nursing, University of Kansas, Mail Stop 4043, 3901 Rainbow Blvd., Kansas City, KS 66160.
J Pre Clin Clin Res. 2010;4(1):5–10
During resuscitation of hemorrhagic shock (HS), clinicians employ high fractions of inspired oxygen (FIO2) to restore maximal oxygen (O2) saturations. Studies indicate that increased FIO2 can be detrimental to cellular function. Our purpose was to determine the FIO2 with and without dopamine (DA) that minimizes hydrogen peroxide (H2O2) production and apoptosis in lung and diaphragm following HS. Sprague-Dawley rats were randomized to FIO2 groups: 0.21, 0.40, 0.60 and 1.0. Controlled HS was elicited by reducing mean arterial pressure to approx. 40 mm Hg. The rats were treated with various FIO2s, with and without DA infusion (10 mcg/kg/min). Hydrogen peroxide was measured using dihydrofl uorescein diacetate. Apoptosis was determined based on nuclear diff erential dye up-take. Compared to 0.21, lung and diaphragm H2O2 and apoptosis were signifi cantly reduced in the 0.40 and 0.60 groups. At an FIO2 of 1.0, H2O2 and apoptosis were greater than at 0.21. With the exception of an FIO2 of 0.40, infusing DA with various FIO2s resulted in H2O2 and apoptosis being signifi cantly decreased. These results indicate that lung and diaphragm H2O2 and apoptosis are aff ected by inspired O2 and DA. Results indicate using an FIO2 of 0.40, with or without DA, is most benefi cial in attenuating tissue damage following HS.
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