No effect of agmatine on the protective activity of clobazam and pregabalin against maximal electroshock-induced seizures in mice
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Department of Pathophysiology, Medical University, Lublin, Poland
Isobolographic Analysis Laboratory, Institute of Agricultural Medicine, Lublin, Poland
Department of Experimental and Clinical Pharmacology, Medical University, Lublin, Poland
Jarogniew J. Łuszczki   

Department of Pathophysiology, Medical University, Jaczewskiego 8, 20-090 Lublin, Poland.
J Pre Clin Clin Res. 2010;4(1):36–39
The aim of this study was to assess the influence of agmatine (AGM – an endogenous neuromodulator / neurotransmitter in the brain) on the protective action of two antiepileptic drugs (clobazam [CLB] and pregabalin [PGB]) in the mouse maximal electroshock seizure (MES) model. Results indicate that AGM at a dose of 100 mg/kg, i.p., 45 min before the test had no significant effect on the anticonvulsant action of CLB or PGB in the MES test in mice. Moreover, the examined combination of AGM with CLB and PGB (at doses from the MES test) did not affect motor coordination in the chimney test and muscular strength in the grip-strength test in mice, indicating no acute adverse effects in animals. Based on the results from this study, it can be concluded that the lack of effect of AGM on the anti-convulsant action of CLB and PGB in the mouse MES model associated with no acute adverse effects in animals, make the combinations of AGM with CLB and PGB neutral from a preclinical point of view.
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