Interaction between diuretics and tiagabine in the maximal electroshock seizure threshold test in mice
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Department of Physiopathology, Institute of Agricultural Medicine, Lublin, Poland
Poland; Department of Pathophysiology, Medical University, Lublin, Poland
J Pre Clin Clin Res. 2010;4(1):40–42
It has been reported that loop diuretics can aff ect the antiseizure action of valproate (VPA) and topiramate (TPM) in mice. This study evaluated the effect of ethacrynic acid (EA), a loop diuretic, and hydrochlorothiazide (HCTZ), a thiazide-type diuretic, on the anticonvulsant activity of tiagabine (TGB), a newer antiepileptic drug, in the maximal electroshock seizure threshold test. The threshold for electroconvulsions is regarded as an experimental model of tonic-clonic seizures in humans. Diuretics were administered intraperitoneally (i.p.) at single doses (acute treatment) or once daily for seven days (chronic administration). EA at subthreshold doses of 12.5 mg/kg (chronic) and 100 mg/kg (acute) did not affect the anticonvulsant activity of TGB. Similarly, HCTZ at the subthreshold dose of 100 mg/kg, both in acute and chronic experiments, remained without effect on the anticonvulsant action of TGB. From the preclinical point of view, the use of tested diuretics in epileptic patients receiving TGB is presumed neutral upon its anticonvulsant potency.
Krzysztof Łukawski   
Department of Physiopathology, Institute of Agricultural Medicine, Jaczewskiego 2, 20-090 Lublin, Poland.
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