RESEARCH PAPER
Impact of smoking status on particular genetic polymorphisms associations with cardiovascular diseases
 
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Department of Internal Medicine, Medical University of Lublin, Poland
CORRESPONDING AUTHOR
Grzegorz Dzida   

Department of Internal Medicine, Medical University of Lublin, Staszica 16, 20-081 Lublin, Poland.
 
J Pre Clin Clin Res. 2012;6(1):31–34
KEYWORDS
ABSTRACT
Background:
Genes encoding : angiotensin converting enzyme (ACE), platelet glycoprotein IIb/IIIa (GP IIb/IIIa) and endothelial nitric oxide synthase (eNOS) meet the criteria for a candidate gene for cardiovascular disease, including myocardial infarction(MI). Myocardial infarction results from an interplay of both genetic and environmental factors. Certain genetic polymorphisms seem to modify the deletorious impact of environmental factors, such as cigarette smoking, and could modify the inherited risk. The aim of the presented study was to assess the influence of cigarette smoking on the incidence of myocardial infarction in the presence of particular ACE, GPIIb/IIIa and eNOS genes polymorphic variants and genotypes.

Material and Methods:
166 individuals of Polish origin were genotyped, all of whom suffered from MI, or had survived it – 138 smokers and 28 non-smokers, all under 55 years of age. Polymorphisms were detected using PCR-RLFP method.

Results:
Genotypes including I allele of ACE gene I/D polymorphism (II and ID) occurred more frequently in smokers with MI compared to non-smokers (p=0.013). Similar findings were observed with T allele of the eNOS G894T polymorphism, and occurred more frequently among smokers when compared to non-smokers (p=0.039). The frequencies of A2 variant of the GPIIb/IIIa PL A1/A2 polymorphism were similar in smoking and non-smoking myocardial interaction patients.

Conclusions:
Cigarette smoking seems to have an impact on the associations between myocardial infarction with ACE gene I/D and eNOS gene G894T polymorphisms, but no interaction was observed with GPIIb/IIIa gene PLA1/A2 plymorphism.

 
REFERENCES (21)
1.
Ichiki K, Ikeda H, Haramaki N, Ueno T, Imaizumi T. Long-term smoking impairs platelet-derived nitric oxide release. Circulation. 1996; 94(12): 3109-3114.
 
2.
Rigat B, Hubert C, Corvol P, Soubrier F. PCR detection of the insertion polymorphism of the humane angiotensin-converting enzyme gene (DCP1) (dipeptidyl carboxypeptidase 1). Nucleic Acid Res. 1992; 20: 1433.
 
3.
Rigat B, Hubert C, Alhenc-Gelas F, Cambien F, Corvol P, Soubrier F. An insertion/deletion polymorphism of the angiotensin I-converting enzyme gene accounting for half the variance of the serum enzyme levels. J Clin Invest. 1990; 86 (4): 1343-6.
 
4.
Wiwanitkit V. PLA1/A2 polymorphism of the platelet glycoprotein receptor IIb/IIIa and its correlation with myocardial infarction: an appraisal., Clin Appl Thromb Hemost. 2006; 12 (10): 93-95.
 
5.
Marsden PA. Structure and chromosomal localisation of the human constitutive endothelial nitric oxide synthase gene. J Biol Chem. 1993; 268: 17478-17488.
 
6.
Sobstyl J, Dzida G, Puźniak A, Mosiewicz J, Hanzlik J. Analiza związku polimorfizmu genu środbłonkowej syntetazy tlenku azotu z zawałem mięśnia serca. Kardiol Pol. 2002; 73: 10-13.
 
7.
Shimasaki Y, Yasue H, Yoshimura M, Nakayama M, Kugiyama K, Ogawa H et al. Association of the missense Glu298Asp variant of the endothelial nitric oxide synthase gene with myocardial infarction. J Am Coll Cardiol. 1998; 31(7): 1506-1510.
 
8.
Gardemann A, Lohre J, Cayci S, Katz N, Tillmanns H, Haberbosch W. The T allele of the missense Glu(298)Asp endothelial nitric oxide synthase gene polymorphism is associated with coronary heart disease in younger individuals with high atherosclerotic risk profile. Atherosclerosis. 2002; 160(1): 167-175.
 
9.
Wang XL, Sim AS, Badenhop RF, McCredie RM, Wilcken DE. A smoking-dependent risk of coronary artery disease associated with a polymorphism of the endothelial nitric oxide synthase gene. Nat Med. 1996; 2(1): 41-45.
 
10.
Madisen L, Hoar DI, Holroyd CD, Crisp M, Hodes ME. DNA banking: the effect of storage of blood and isolated DNA on integrity of DNA, Am J Med Genet. 1987; 27(2): 379-390.
 
11.
Odawara M. Mistyping frequency of the angiotensin-converting enzyme gene polymorphism and improved method for its avoidance. Hum Genet. 1997; 100: 163.
 
12.
Arias-Vasquez A, Sayed-Tabatabaei FA, Schut AF, Hofman A, Bertolli- Avella AM, Vergeer JM, et al. Angiotensin converting enzyme gene, smoking and mortality in a population-based study. Eur J Clin Invest. 2005; 35(7): 444-449.
 
13.
Sayed-Tabatabaei FA, Schut AF, Arias Vasquez A, Bertoli-Avella AM, Hofman A, Witteman JC, et al. Angiotensin converting enzyme gene polymorphism and cardiovascular morbidity and mortality: the Rotterdam Study. J Med. Genet. 2005; 42(1): 26-30.
 
14.
Espinosa JS, Rueda E, Munoz E, Montiel A, Martinez S, Dieguez JL et al. Association between myocardial infarction and angiotensin converting enzyme gene polymorphism in young patients. Med. Clin (Barc). 1998; 110 (13): 488-491.
 
15.
Butler R, Morris AD, Burchell B, Struthers AD. DD angiotensinconverting enzyme gene polymorphism is associated with endothelial dysfunction in normal humans. Hypertension. 1999; 33(5): 1164-1168.
 
16.
Weiss EJ, Bray PF, Tayback M, Schulman SP, Kickler TS, Becker LC, et al. A polymorphism of a platelet glycoprotein receptor as an inherited risk factor for coronary thrombosis., N Engl J Med. 1996; 334: 1090-1094.
 
17.
Grove EL, Orntoft TF, Lassen JF, Jensen HK, Kristensen SD. The platelet polymorphism PLA2 is a genetic risk factor for myocardial infarction. J Inter Med. 2004; 255 (6): 637-44.
 
18.
Lopes NH, Pereira AC, Hueb W, Soares PR, Lanz JR, Gersh BJ, et al. Effect of glycoprotein IIIa PLA2 polymorphism on outcome of patients with stable coronary disease and effect of smoking. Am J Cardiol. 2004; 93 (12): 1469-1472.
 
19.
Ardissino D, Mannucci PM, Merlini PA, Duca F, Fetiveau R, Tagliabue L, et al. Prothrombotic genetic risk factors in young survivors of myocardial infarction. Blood. 1999; 94: 46-51.
 
20.
Leeson CP, Hingorani AD, Mullen MJ, Jeerooburkhan N, Kattenhorn M, Cole TJ, et al. Glu298Asp endothelial nitric oxide synthase gene polymorphism interacts with environmental and dietary factors to influence endothelial function. Circ Res. 2002; 90: 1153-1158.
 
21.
Chang K, Baek SH, Seung KB, Kim PJ, Ihm SH, Chae JS, et al. The Glu298Asp polymorphism in the endothelial nitric oxide synthase gene is strongly associated with coronary spasm. Coron Artery Dis. 2003; 14: 293-299.
 
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