Hematological effects of protein kinases inhibitor maleimide derivative (1-(4 -Cl-benzyl)-3-Cl-4-(CF3-phenylamino)-1H-pyrrole-2,5-dione)
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Department of Cytophysiology, Taras Shevchenko National University, Kiev, Ukraine
Department of Hematology, Research Centre for Radiation Medicine Academy of Medical Science of Ukraine
Iryna Byelinska   

Cytophysiology Department, Faculty of Biology, 2, Academician Glushkov Avenue, Building 12, Taras Shevchenko National University, Kiev, Ukraine.
J Pre Clin Clin Res. 2010;4(1):32–35
Maleimide derivative (MD, 1-(4 -Cl-benzyl)-3-Cl-4-(CF3-phenylamino)-1Н-pyrrole-2,5-dione) is an inhibitor of a number of protein kinases and proliferative activity of tumour cells. A study of potential anticancer drug effects on different systems of the organism, including the hemopoietic system, is an urgent issue. The aim of the present study was to investigate MD eff ects on rat blood cells parameters. It was found that daily treatment with MD for 25 days (2.7, 0.027, 0.00027 mg/kg) does not signifi cantly affect the erythroid parameters of rats. However, MD at 2.7 and 0.027 mg/kg slightly increased and at 0.00027 mg/kg decreased the erythrocytes mean volume. MD at 0.00027 mg/kg did not affect the number of blood leukocytes. MD at 2.7 and 0.027 mg/kg reduced the leukocytes count by decreasing the absolute number of the neutrophylic and eosinophylic granulocytes and monocytes. Thus, the MD-induced effects on the erythrocytes and thrombocytes parameters do not restrict its application as an antitumor drug in indicated doses. However, it is necessary to control the leukocytes count to detect the occurrence of leukopenia during MD therapy.
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