RESEARCH PAPER
Characteristics of the premature phase 3 of the migrating motor complex evoked by pirenzepine administration in the small bowel of non-fasted sheep
 
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Department of Biostructure and Animal Physiology, Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wrocław, Poland
CORRESPONDING AUTHOR
Krzysztof Romański   

Department of Biostructure and Animal Physiology, Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Norwida 31, 50-375 Wrocław, Poland.
 
J Pre Clin Clin Res. 2010;4(1):47–51
KEYWORDS
ABSTRACT
In sheep, pirenzepine (Pi) blocks muscarinic receptors thus inhibiting the gastrointestinal motility. However, its small doses also evoke the premature phase 3 of the migrating motor complex (MMC). The aim of this study was to specify further the character of stimulatory alterations caused by the drug in non-fasted sheep. The experiments were carried out in four conscious rams with sewn duodenal strain gauge force transducer and duodeno-jejunal bipolar electrodes. Following the control period, 0.15 M NaCl or Pi, at the dose 0.02 mg/kg body weight, were slowly administered into the jugular vein and motor-myoelectric activity recordings were continued until normal motility returned. In the course of most experiments Pi evoked the premature phase 3 preceded by 3-5 min inhibition in non-fasted rams. Its duration in the duodenum was shorter than in the jejunum and the same difference was observed in normal phase 3 of the MMC. Amplitude and duration of the spike bursts forming the premature phase 3 was not significantly different from normal phase 3 of the MMC cycle. The premature phase 3 exhibited a regularly propagating character in most cases, but occurred also in incomplete or disorganized forms. Usually, its character thus closely resembled the normal phase 3 of the MMC. It appears that Pi, at the low doses, triggers the premature phase 3 via the blockade of neuronal M1 receptor subtype that provokes the secondary activation of acetylcholine release. Induction of premature phase 3 by a small dose of Pi resembles the effect observed in monogastric species.
 
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