Biochemical bone metabolism markers and morphometric, densitometric and biomechanical properties of femur and tibia in female and gonadectomised male Polish Landrace pigs
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Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland
II Department of Radiology, Medical University, Lublin, Poland
Department of Conservative Dentistry, Medical University, Lublin, Poland
Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland
J Pre Clin Clin Res. 2012;6(1):14–19
Estrogens and androgens are critical regulators of bone metabolism and maintain bone mass throughout live in humans and animals. The aim of the presented study was to compare biochemical bone turnover markers and hormones influencing bone tissue metabolism and skeletal properties in female and orchidectomised male pigs at slaughter age. To achieve this aim, femur and tibia from 6-month-old pigs were investigated in terms of morphometric, densitometric and biomechanical properties. Serum evaluation of osteocalcin (OC), bone-specific alkaline phosphatase (BAP), growth hormone (GH), insulin-like growth factor-1 (IGF-1) and cotrisol in newborn and 90-day-old pigs was performed. This study shows a significantly higher growth rate and final body weight gain in orchidectomised males when compared to females (p<0.05). The obtained results have not shown statistically significant differences in weight, length, volumetric bone mineral density of the trabecular and cortical bone, vertical and horizontal diameters of the mid-shaft, cross-sectional area, second moment of inertia, mean relative wall thickness, cortical index, maximum elastic strength and ultimate strength of femur and tibia in females and orchidectomised males (p>0.05). Bone formation markers such as BAP and OC assessed in serum were not significantly different between both the investigated groups (p>0.05). Furthermore, serum concentrations of GH, IGF-1 and cortisol were not gender-differentiated (p>0.05). In conclusion, the differences in body weight gain were not influenced by gender-differentiated serum concentrations of GH, IGF-1 and cortisol. Lack of significant differences of morphological, densitometric and biomechanical properties of femur and tibia between the groups was related to very similar levels of OC, BAP, GH, IGF-1 and cortisol. The data obtained provide novel physiological information on bone metabolism markers and regulators, as well as properties of femur and tibia in female and gonadectomised male pigs. The results obtained in this study may therefore be useful in experimental approaches for studying the effects of physiological, nutritional, pharmacological, toxicological and environmental factors on bone metabolism and skeletal system properties in pigs.
Marcin R. Tatara   
Department of Animal Physiology, University of Life Sciences, Akademicka 12, 20-950 Lublin, Poland.
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