RESEARCH PAPER
Prandial and postprandial exocrine pancreatic secretion after duodenal infusion of alpha-ketoglutarate, formic acid and potassium di-formate in pigs
 
More details
Hide details
1
Department of Cell and Organism Biology, Lund University, Lund, Sweden
2
Institute of Agricultural Medicine, Lublin, Poland
3
Department of Animal Biochemistry and Physiology, Faculty of Veterinary Medicine, Agricultural University, Lublin, Poland
CORRESPONDING AUTHOR
Stefan Pierzynowski   

Department of Cell and Organism Biology, Lund University, Helgonavagen 3b, 223 62 Lund, Sweden.
 
J Pre Clin Clin Res. 2007;1(1):60–64
KEYWORDS
ABSTRACT
The positive effect of acidifiers on performance (growth) may be associated with the stimulation of the pancreatic secretion since some studies have shown a direct relationship between pancreatic enzyme outflow and body weight gain and feed conversion. The aim of the study was to investigate the pancreatic juice (PJ) composition after treatment with alpha-ketoglutarate (AKG), formic acid (FA) and its potassium salt (KDF). Experiments were conducted on 9 pigs (15 ± 5 kg. bw.) surgically fitted with a pancreatic duct catheter, a T-cannula in the duodenum and a jugular vein catheter. The piglets were fed a standard diet twice daily and had free access to tap water. During the prandial period, duodenal infusions of KDF, FA, AKG or saline 0.9% NaCl (2.5ml/h/kg bw.) were carried out in experimental and control pigs, respectively. PJ was analyzed for volume, protein content and trypsin activity, and blood assayed for cholecystokinin (CCK). Both AKG and KDF had stimulatory effects on the PJ outflow. The PJ volume increased significantly in the prandial phase (p<0.05) in the AKG group, and increased significantly in both the prandial and postprandial phases (p<0.05) in the KDF group. Formic acid did not stimulate exocrine pancreatic secretion. There were no effects on protein and trypsin output and plasma CCK levels after the treatment. In conclusion, the exocrine pancreatic secretion can be stimulated by oral supplementation with AKG and KDF, and this effect is mediated mainly by secretin since only volume outflow was altered in the pigs.
 
REFERENCES (15)
1.
Pierzynowski SG, Westrom BR, Svendsen J, Karlsson BW: Development of exocrine pancreas function in chronically cannulated pigs during 1-13 weeks of postnatal life. JPEN 1990, 10, 206-212.
 
2.
Pierzynowski SG, Westrom BR, Karlsson BW, Svendsen J, Svendsen L: Development and regulation of porcine pancreatic function. Int J Pancreatology 1995, 18(2), 81-94.
 
3.
Cranwell PD: The development of acid and pepsin (EC 3.4.23.1 secretory capacity in the pig; the effects of age and weaning. suckling pig. Br J Nutr 1985, 54, 305-320.
 
4.
Roth FX, Kirchgessner M: Organic acids as feed additives for young pig: Nutritional and gastrointestinal effects. J Anim Feed Sci 1998, 7, 25-33. Lueck E: Antimicrobial food additives: characteristics, uses, effects. Berlin, Springer Verlag, 1980.
 
5.
Kirchgessner M, Roth FX, Paulicks BR: Zur nutritiven Wirkung von Sorbinsäure in der ferkelaufzucht. J Anim Physiol An Nr 1995, 74, 235- 242.
 
6.
Raul F, Galluser-Goose F, Galluser M, Hasselmann M, Seiler N: Functional and metabolic changes in intestinal mucosa of rats after enteral adminstration of Ornithine Alpha ketoglutarate. JPEN 1995, 19, 145-150.
 
7.
Thaela MJ, Hedemann MS, Jensen BB, Jakob S, Pierzynowski SG, Jensen MS: Effect of supplementation of lactic acid on the pancreatic secretion in pigs after weaning. Antinutrients – for better or for worse. Interaction with intestinal physiology and microbiology, Cost 98, Tromso, June 18-19, 1998.
 
8.
Botemans JAM and Pierzynowski SG: Relations between body weight, feed intake, daily weight gain, and exocrine pancreatic secretion in chronically catheterized growing pigs. J Anim Sci, 1999, 77, 450-456.
 
9.
Lowry OH, Roseebrough N, Farr A, Randall RJ: Protein measurement with the folin phenol reagent. J Biol Chem 1951, 193, 265-275.
 
10.
Erlanger BF, Fedel F, Cooper AG: The action of chymotrypsin on two new chromogenic substrates. Arch Biochem Biophys 1966, 115, 206- 210.
 
11.
Tschierschwitz A, Grassmann E, Kirchgessner M, Roth FX: The effect of fumaric acid supplements on activities of liver enzymes (GOT , GPT, SUCCDH ) with different supplies of energy and protein to growing rats) Zeitschrift fur Tierphysiologie, Tierernahrung und Futtermittlkunde 1982, 48, 253-259.
 
12.
Grassmann E, Kirchgessner M: Absorption of copper from complexes with various organic acids, Zeitschrift fur Tierphysiologie, Tierernahrung und Futtermittlkunde 1979, 25, 125-128.
 
13.
Kirchgessner M, Roth FX: Energy value of organic acids in the rearing of piglets and the fattening of pigs. Übersichten zur Tierernahrung 1988, 16, 93-108.
 
14.
Singer MV: Pancreatory secretory response to intestinal stimulants: A review. Scand J Gastroentrol 1987, 22, 1-135.
 
15.
Pierzynowski SG, Martensson H, Westrom BR, Ahrén B, Uvnäs-Moberg K, Karlsson BW: Cholecytokinin (CCK - 33) can stimulate pancreatic secretion by a local intestinal mechanism in the pig. Biomed Res 1993, 14, 217-221.
 
eISSN:1898-7516
ISSN:1898-2395