Prandial and postprandial exocrine pancreatic secretion after duodenal infusion of alpha-ketoglutarate, formic acid and potassium di-formate in pigs
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Department of Cell and Organism Biology, Lund University, Lund, Sweden
Institute of Agricultural Medicine, Lublin, Poland
Department of Animal Biochemistry and Physiology, Faculty of Veterinary Medicine, Agricultural University, Lublin, Poland
Stefan Pierzynowski   

Department of Cell and Organism Biology, Lund University, Helgonavagen 3b, 223 62 Lund, Sweden.
J Pre Clin Clin Res. 2007;1(1):60–64
The positive effect of acidifiers on performance (growth) may be associated with the stimulation of the pancreatic secretion since some studies have shown a direct relationship between pancreatic enzyme outflow and body weight gain and feed conversion. The aim of the study was to investigate the pancreatic juice (PJ) composition after treatment with alpha-ketoglutarate (AKG), formic acid (FA) and its potassium salt (KDF). Experiments were conducted on 9 pigs (15 ± 5 kg. bw.) surgically fitted with a pancreatic duct catheter, a T-cannula in the duodenum and a jugular vein catheter. The piglets were fed a standard diet twice daily and had free access to tap water. During the prandial period, duodenal infusions of KDF, FA, AKG or saline 0.9% NaCl (2.5ml/h/kg bw.) were carried out in experimental and control pigs, respectively. PJ was analyzed for volume, protein content and trypsin activity, and blood assayed for cholecystokinin (CCK). Both AKG and KDF had stimulatory effects on the PJ outflow. The PJ volume increased significantly in the prandial phase (p<0.05) in the AKG group, and increased significantly in both the prandial and postprandial phases (p<0.05) in the KDF group. Formic acid did not stimulate exocrine pancreatic secretion. There were no effects on protein and trypsin output and plasma CCK levels after the treatment. In conclusion, the exocrine pancreatic secretion can be stimulated by oral supplementation with AKG and KDF, and this effect is mediated mainly by secretin since only volume outflow was altered in the pigs.
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